Levenkova Natasha, Gu Qingjuan, Rux John J
The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA.
Bioinformatics. 2004 Feb 12;20(3):430-2. doi: 10.1093/bioinformatics/btg437. Epub 2004 Jan 22.
Small interfering RNA (siRNA) is used in functional genomics applications to decrease the expression of a target gene, which may yield a biological effect that suggests a function for the target gene. The siRNA design tool scans a target gene for candidate siRNA sequences that satisfy user-adjustable rules. Selected candidates are then screened to identify those siRNA sequences that are specific to the gene of interest.
小干扰RNA（siRNA）用于功能基因组学应用，以降低靶基因的表达，这可能会产生一种生物学效应，提示该靶基因的功能。siRNA设计工具会在靶基因中扫描符合用户可调整规则的候选siRNA序列。然后对选定的候选序列进行筛选，以鉴定出那些对目标基因具有特异性的siRNA序列。
