Ottiger C, Regeniter A, Köchli H P, Huber A R
Zentrum für Labormedizin, Kantonsspital Aarau.
Praxis (Bern 1994). 2004 Jan 7;93(1-2):15-21. doi: 10.1024/0369-8394.93.1.15.
We compared the number of particles in the urine with flow cytometry and manual methods: cell chamber and standardised sediment analysis. The correlation (r) between the KOVA-cell chamber and the flow cytometer UF-100 was 0.966 for erythrocytes, 0.935 for leukocytes and 0.902 for squamous epithelial cells. Similar results were obtained by a standardised preparation of the sediment. Today, the estimation of the cell number in the sediment analysis is still common. The KOVA cell chamber system is a cheap alternative for microscopy, whereas automation with flow cytometry is only used for large laboratories. Reference values were established under optimal conditions (erythrocytes < 14/microliter, leukocytes < 16/microliter) with a cut-off of 20/microliter.
我们使用流式细胞术和手工方法（细胞计数板和标准化沉渣分析）比较了尿液中的颗粒数量。KOVA细胞计数板与流式细胞仪UF-100之间，红细胞的相关性（r）为0.966，白细胞为0.935，鳞状上皮细胞为0.902。通过标准化的沉渣制备也获得了类似的结果。如今，沉渣分析中的细胞数量估算仍然很常见。KOVA细胞计数板系统是一种用于显微镜检查的廉价替代方法，而流式细胞术自动化仅用于大型实验室。在最佳条件下（红细胞<14/微升，白细胞<16/微升）建立了参考值，临界值为20/微升。
