Omarov Rustem T, Rezende Jorge A M, Scholthof Herman B
Department of Plant Pathology and Microbiology, Texas A&M University, College Station 77843, USA.
Mol Plant Microbe Interact. 2004 Feb;17(2):195-201. doi: 10.1094/MPMI.2004.17.2.195.
The accumulation of Tomato bushy stunt virus (TBSV) defective interfering RNAs (DIs) has been observed in several species of plants, but the involvement of host-specific processes and the functional role of DIs are still poorly understood. In this study, the accumulation of DIs was compared after several passages of TBSV through Nicotiana benthamiana and pepper (Capsicum annuum). As anticipated, passages of wild-type TBSV through N. benthamiana resulted in the accumulation of significant levels of TBSV DIs, which caused symptom attenuation and prevented the plants from lethal necrosis. On the contrary, TBSV infection of pepper plants caused severe local and systemic chlorosis, but continuous virus passages did not result in detectable levels of DIs accumulation. In addition, the inoculation of pepper plants with a mixture of helper virus and DI either from in vitro generated transcripts or from infected N. benthamiana did not yield DI in upper pepper leaves. Our cumulative results suggest that complex host-specific determinants play an important role in TBSV DI generation and their subsequent maintenance and accumulation.
番茄丛矮病毒（TBSV）缺陷干扰RNA（DIs）已在多种植物中被观察到积累，但宿主特异性过程的参与情况以及DIs的功能作用仍知之甚少。在本研究中，比较了TBSV在本氏烟草和辣椒（辣椒属）中传代几次后DIs的积累情况。正如预期的那样，野生型TBSV在本氏烟草中传代导致大量TBSV DIs积累，这导致症状减轻并防止植物发生致死性坏死。相反，辣椒植株感染TBSV会导致严重的局部和系统性褪绿，但病毒的连续传代并未导致可检测水平的DIs积累。此外，用辅助病毒和来自体外转录物或受感染本氏烟草的DIs混合物接种辣椒植株，在上部辣椒叶片中未产生DIs。我们的累积结果表明，复杂的宿主特异性决定因素在TBSV DI的产生及其随后的维持和积累中起重要作用。
