大鼠大脑皮质突触后致密物中微管相关蛋白（MAP）1B的定位
Localization of microtubule-associated protein (MAP) 1B in the postsynaptic densities of the rat cerebral cortex.
作者信息
Kawakami Shin-Ichi, Muramoto Kazuyo, Ichikawa Masumi, Kuroda Yoichiro
机构信息
School of Agricultural Sciences, Nagoya University, Chigusa, Nagoya, Japan.
出版信息
Cell Mol Neurobiol. 2003 Dec;23(6):887-94. doi: 10.1023/b:cemn.0000005317.79634.27.
Abstract
- Although microtubule-associated protein (MAP) 1B and its phosphorylation have been suggested to be important for synapse formation among cortical neurons, the localization of MAP1B in synapses has not yet been confirmed. In this report, we examine the localization of MAP1B in synaptic regions. 2. The localization of MAP1B was observed by immunohistochemical and electron microscopic techniques using specific antibodies against MAP1B. 3. MAP1B immunoreactivities were widely distributed in the cerebral cortex and were observed in the postsynaptic area but not in presynaptic terminals. 4. These synapses were classified as the asymmetrical type. 5. Only some synapses exhibited MAP1B immunoreactivities. MAP1B-immunopositive synapses accounted for about half of the total synapses. 6. Such a localization suggests MAP1B's important roles in synaptic functions.
摘要
- 尽管微管相关蛋白（MAP）1B及其磷酸化作用被认为对皮质神经元之间的突触形成很重要，但MAP1B在突触中的定位尚未得到证实。在本报告中，我们研究了MAP1B在突触区域的定位。2. 使用针对MAP1B的特异性抗体，通过免疫组织化学和电子显微镜技术观察MAP1B的定位。3. MAP1B免疫反应广泛分布于大脑皮层，在突触后区域可见，但在突触前终末未见。4. 这些突触被归类为不对称型。5. 只有一些突触表现出MAP1B免疫反应。MAP1B免疫阳性突触约占突触总数的一半。6. 这种定位表明MAP1B在突触功能中起重要作用。
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