Zhao Ning, Yang Pi-shan, Ge Shao-hua, Qi Xiang-min, Sun Qin-feng
School of Stomatology, Shandong University. Jinan 250012, China.
Shanghai Kou Qiang Yi Xue. 2003 Aug;12(4):269-72.
To explore the biological effects of tetracycline and doxycycline on cultured human periodontal ligament cells(HPDLCs).
Increasing concentrations of two drugs(1,5,20,100,500,2500 microg/ml)were added to the medium of cultured HPDLCs separately. After being co-incubated for 2 days, cell morphology was observed under reverse microscope; meanwhile, cell proliferation activity was assayed using MTT, the total amount of protein was detected by Coumassie Bright Blue method and DNA synthesis was measured by 3H-TdR.
Over a concentration range of 1 to 100 microg/ml, cell morphology was normal. Moreover, at a concentration range of 20 to 100 microg/ml, tetracycline significantly enhanced the proliferative activity and biosynthesis of HPDLCs (P<0.01) while doxycycline significantly promoted DNA synthesis only at the concentration of 20 microg/ml. However, higher concentration of the two drugs (2500 microg/ml) not only changed cell morphology under microscope, but also significantly inhibited cellular activity.
The results suggested that proper doses of tetracycline could promote proliferation and biosynthesis of HPDLCs,while doxycycline had limited effects on biobehavior of HPDLCs. Nevertheless, higher concentrations of the two drugs both had cytotoxic effect.
探讨四环素和多西环素对培养的人牙周膜细胞（HPDLCs）的生物学作用。
将两种药物浓度递增（1、5、20、100、500、2500微克/毫升）分别加入培养的HPDLCs培养基中。共孵育2天后，在倒置显微镜下观察细胞形态；同时，采用MTT法检测细胞增殖活性，考马斯亮蓝法检测蛋白质总量，3H-TdR法检测DNA合成。
在1至100微克/毫升的浓度范围内，细胞形态正常。此外，在20至100微克/毫升的浓度范围内，四环素显著增强HPDLCs的增殖活性和生物合成（P<0.01），而多西环素仅在20微克/毫升浓度时显著促进DNA合成。然而，两种药物的高浓度（2500微克/毫升）不仅改变显微镜下的细胞形态，还显著抑制细胞活性。
结果表明，适当剂量的四环素可促进HPDLCs的增殖和生物合成，而多西环素对HPDLCs的生物学行为影响有限。然而，两种药物的高浓度均具有细胞毒性作用。
