Xu Yan-Zhi, Zou Hui-Ru, Wang Xiao-Ling, Liu Shi-Zheng, Wang Yong-Jun
Department of Stomatology, The Fourth Hospital, Hebei Medical University, Shijiazhuang 050011, China.
Chin Med J (Engl). 2004 Nov;117(11):1693-6.
Successful periodontal regeneration depends on the migration, proliferation and differentiation of periodontal ligament cells in periodontal defects. The total protein content and the ultrastructure demonstrate the metabolizability and activity of periodontal ligament cells. This study was conducted to observe the effects of Shuanghuangbu, a mixture of medicinal herbs, on the total protein content and the ultrastructure of human periodontal ligament cells.
Periodontal ligament cells were grown to confluence and then cultured in Dulbecco's modified eagle medium (DMEM) supplemented with Shuanghuangbu over the concentration range of 0 to 1000 microg/ml. The total protein content in cultured cells was determined by using Coommasie brilliant blue technique. Periodontal ligament cells were incubated in 0 and 100 microg/ml Shuanghuangbu decoction for 5 days, then observed through transmission electron microscope.
The total protein content of human periodontal ligament cells increased in each experiment group added 10 - 1000 microg/ml Shuanghuangbu respectively, and the effect of 100 microg/ml was excellent. Under the transmission electron microscope, there were more rough endoplasmic reticulums and mitochodrias in the experiment group than those in the control group.
Shuanghuangbu stimulates the protein synthesis of human periodontal ligament cells and improves human periodontal ligament cells' metabolizability and activity.
牙周组织的成功再生取决于牙周膜细胞在牙周缺损处的迁移、增殖和分化。牙周膜细胞的总蛋白含量和超微结构反映了其代谢能力和活性。本研究旨在观察中药复方双黄补对人牙周膜细胞总蛋白含量及超微结构的影响。
将牙周膜细胞培养至汇合后，在添加浓度为0至1000微克/毫升双黄补的杜氏改良 Eagle 培养基（DMEM）中培养。采用考马斯亮蓝法测定培养细胞中的总蛋白含量。将牙周膜细胞分别在含0和100微克/毫升双黄补水煎剂的条件下孵育5天，然后通过透射电子显微镜观察。
在分别添加10 - 1000微克/毫升双黄补的各实验组中，人牙周膜细胞的总蛋白含量均增加，其中100微克/毫升组效果最佳。透射电子显微镜下观察发现，实验组细胞中的粗面内质网和线粒体比对照组更多。
双黄补可促进人牙周膜细胞的蛋白质合成，提高人牙周膜细胞的代谢能力和活性。
