Creeper F, Lichanska A M, Marshall R I, Seymour G J, Ivanovski S
School of Dentistry, The University of Queensland, Queensland, Australia.
J Periodontal Res. 2009 Apr;44(2):258-65. doi: 10.1111/j.1600-0765.2008.01125.x. Epub 2009 Feb 6.
Platelet-rich plasma is used to deliver growth factors, in a safe and convenient manner, for enhancing bone and periodontal regeneration. However, conflicting reports regarding its effectiveness suggest that further study of the relevant cellular mechanisms is required. The aim of this study was to investigate the in vitro effect of platelet-rich plasma on osteoblasts and periodontal ligament cell function.
Various concentrations of platelet-rich plasma (100, 50, 20 and 10%) and platelet-poor plasma, obtained from human donors, were applied to primary cultures of human osteoblasts and periodontal ligament cells. [(3)H]-Thymidine incorporation, crystal violet staining and MTT assays were utilized to assess DNA synthesis and proliferation. Migration was determined by assessing the cell response to a concentration gradient, while differentiation was assessed using Alazarin Red staining.
Platelet-rich plasma and platelet-poor plasma had stimulatory effects on the migration of both human osteoblasts and periodontal ligament cells. At 24 h, DNA synthesis was suppressed by the application of the various concentrations of platelet-rich plasma, but over a 5-d period, a beneficial effect on proliferation was observed, especially in response to 50% platelet-rich plasma. Platelet-poor plasma resulted in the greatest enhancement of cellular proliferation for both cell types. At a concentration of 50%, platelet-rich plasma and platelet-poor plasma facilitated differentiation of both cell types.
Platelet-rich plasma can exert a positive effect on osteoblast and periodontal ligament cell function, but this effect is concentration specific with maximal concentrations not necessarily resulting in optimal outcomes. Platelet-poor plasma also appears to have the ability to promote wound healing-associated cell function.
富血小板血浆被用于以安全便捷的方式递送生长因子，以促进骨和牙周组织再生。然而，关于其有效性的报道相互矛盾，这表明需要进一步研究相关的细胞机制。本研究旨在探讨富血小板血浆对成骨细胞和牙周膜细胞功能的体外影响。
从人类供体获取不同浓度（100%、50%、20%和10%）的富血小板血浆和贫血小板血浆，将其应用于原代培养的人成骨细胞和牙周膜细胞。采用[³H]-胸腺嘧啶核苷掺入法、结晶紫染色法和MTT法评估DNA合成和细胞增殖。通过评估细胞对浓度梯度的反应来测定细胞迁移，同时使用茜素红染色评估细胞分化。
富血小板血浆和贫血小板血浆对人成骨细胞和牙周膜细胞的迁移均有刺激作用。在24小时时，不同浓度的富血小板血浆抑制了DNA合成，但在5天的时间里，观察到其对细胞增殖有有益作用，尤其是对50%的富血小板血浆反应明显。贫血小板血浆对两种细胞类型的细胞增殖促进作用最大。在50%的浓度下，富血小板血浆和贫血小板血浆均促进了两种细胞类型的分化。
富血小板血浆可对成骨细胞和牙周膜细胞功能产生积极影响，但这种影响具有浓度特异性，最高浓度不一定能产生最佳效果。贫血小板血浆似乎也有促进与伤口愈合相关的细胞功能的能力。
