Takegawa Yasuhiro, Ito Shinya, Yoshioka Shinji, Deguchi Kisaburo, Nakagawa Hiroaki, Monde Kenji, Nishimura Shin-Ichiro
Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 001-0021, Japan.
Rapid Commun Mass Spectrom. 2004;18(4):385-91. doi: 10.1002/rcm.1341.
Two isomeric pairs of 2-aminopyridine (PA)-derivatized fucosylated and non-fucosylated oligosaccharides (complex-type N-glycans of IgG) were analyzed using liquid chromatography/ion trap mass spectrometry (LC/ITMS) with a sonic spray ionization source and by varying the collision-induced dissociation voltage. Reproducibility of MS(n) (n = 2) spectra obtained by LC/ITMS was tested considering both fragment ions (m/z) and intensities. A comparison of their MS(n) spectra and evaluation of similarities (or matching), based on correlation coefficients between MS(n) spectra, was investigated as a possibility for structural assignment of the isomers. It is shown that such MS(n) spectral matching is useful and applicable to the structural assignment of relatively large fucosylated and sialylated PA-oligosaccharides released from IgG based on Bn- and Yn-type fragmentations of the corresponding M+H+Na ions.
使用配备超声喷雾电离源的液相色谱/离子阱质谱（LC/ITMS）并通过改变碰撞诱导解离电压，分析了两对2-氨基吡啶（PA）衍生的岩藻糖基化和非岩藻糖基化寡糖（IgG的复合型N-聚糖）的异构体。考虑到碎片离子（m/z）和强度，测试了通过LC/ITMS获得的MS(n)（n = 2）光谱的重现性。基于MS(n)光谱之间的相关系数，研究了它们的MS(n)光谱比较和相似性（或匹配）评估，以此作为异构体结构归属的一种可能性。结果表明，这种MS(n)光谱匹配是有用的，并且适用于基于相应M+H+Na离子的Bn型和Yn型裂解从IgG释放的相对较大的岩藻糖基化和唾液酸化PA-寡糖的结构归属。
