Wu Ming-Yao, Zhuang Chu-Xiang, Yang Huan-Xing, Liang Ying-Rui
Department of Pathology, Shantou University Medical College, 22 Xinling Road, Shantou 515031, Guangdong Province, China.
World J Gastroenterol. 2004 Feb 15;10(4):476-80. doi: 10.3748/wjg.v10.i4.476.
To examine the expression of Egr-1, c-fos and cyclin D1 at both transcript and protein levels in esophageal carcinoma and to correlate the level of their expressions with precancerous and paracancerous esophageal lesions and esophageal carcinoma.
In situ hybridization and immunohistochemistry were used respectively to detect the expression of mRNA and proteins of Egr-1, c-fos and cyclin D1 in 70 cases of esophageal squamous cell carcinoma and their corresponding para-cancerous mucosa and upper cut edge mucosa.
In situ hybridization and immunohistochemistry showed positive staining of all three mRNAs in the cytoplasm and those of the proteins in nuclei. Overexpression of Egr-1, c-fos and cyclin D1 mRNAs and their proteins was found in dysplasia and squamous carcinomas. The expression level of Egr-1 and c-fos was high, and cyclin D1 was low in dysplasia mucosa, whereas the expression of Egr-1 was decreased, c-fos was maintained and cyclin D1 was increased in the cancers. The expression of both c-fos and cyclinD1 was consistent between the mRNA and protein in their corresponding high expression lesions.
The expression of Egr-1, c-fos and cyclin D1 varies in esophageal precancerous lesions and cancer tissues, suggesting an involvement of these genes in the development of esophageal carcinoma.
检测早期生长反应基因-1（Egr-1）、原癌基因c-fos和细胞周期蛋白D1（cyclin D1）在食管癌组织及癌旁和癌上切缘组织中的mRNA和蛋白表达水平，并分析其与食管癌及癌前病变的关系。
采用原位杂交和免疫组化方法分别检测70例食管鳞状细胞癌组织及其相应的癌旁黏膜组织和癌上切缘黏膜组织中Egr-1、c-fos和cyclin D1的mRNA和蛋白表达。
原位杂交和免疫组化结果显示，Egr-1、c-fos和cyclin D1的mRNA在细胞浆中呈阳性染色，其蛋白在细胞核中呈阳性染色。在发育异常和鳞状细胞癌组织中均发现Egr-1、c-fos和cyclin D1的mRNA及其蛋白表达上调。在发育异常的黏膜组织中Egr-1和c-fos表达水平较高，cyclin D1表达水平较低；而在癌组织中Egr-1表达下降，c-fos表达维持不变，cyclin D1表达上调。在相应的高表达病变组织中，c-fos和cyclin D1的mRNA和蛋白表达具有一致性。
Egr-1、c-fos和cyclin D1在食管癌前病变和癌组织中的表达存在差异，提示这些基因参与了食管癌的发生发展过程。
