银杏叶提取物对十二指肠溃疡大鼠细胞保护因子的影响。

Effects of Ginkgo biloba extract on cytoprotective factors in rats with duodenal ulcer.

作者信息

Chao Jane C J, Hung Huei-Chen, Chen Sheng-Hsuan, Fang Chia-Lang

机构信息

School of Nutrition and Health Sciences, Taipei Medical University, 250 Wu Hsing Street, Taipei, Taiwan 110, China.

出版信息

World J Gastroenterol. 2004 Feb 15;10(4):560-6. doi: 10.3748/wjg.v10.i4.560.

DOI:10.3748/wjg.v10.i4.560

PMID:14966917

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4716980/

Abstract

AIM

To investigate the effects of Ginkgo biloba extract on cytoprotective factors in rats with duodenal ulcer.

METHODS

Sprague-Dawley rats were randomly divided into four groups: sham operation without ginkgo, sham operation with ginkgo, duodenal ulcer without ginkgo, and duodenal ulcer with ginkgo. Rats with duodenal ulcer were induced by 500 mL/L acetic acid. Rats with ginkgo were intravenously injected with Ginkgo biloba extract from the tail at a dose of 0.5 mg/(kg/d) for 7 and 14 days.

RESULTS

Pathological result showed that duodenal ulcer rats with ginkgo improved mucosal healing and inflammation compared with those without ginkgo after 7 d treatment. After 14 d treatment, duodenal ulcer rats with ginkgo significantly increased weight gain (34.0+/-4.5 g versus 24.5+/-9.5 g, P<0.05) compared with those without ginkgo. Duodenal ulcer rats significantly increased cell proliferation (27.4+/-4.0 and 27.8+/-2.3 BrdU-labeled cells in duodenal ulcer rats with and without ginkgo versus 22.4+/-3.5 and 20.8+/-0.5 BrdU-labeled cells in sham operation rats with and without ginkgo, P<0.05) compared with sham operation rats. Mucosal prostaglandin E(2) concentration significantly increased by 129% (P<0.05) in duodenal ulcer rats with ginkgo compared with that in those without ginkgo. Duodenal ulcer rats without ginkgo significantly decreased superoxide dismutase activity in the duodenal mucosa and erythrocytes (19.4+/-6.7 U/mg protein versus 38.1+/-18.9 U/mg protein in the duodenal mucosa, and 4.87+/-1.49 U/mg protein versus 7.78+/-2.16 U/mg protein in erythrocytes, P<0.05) compared with sham operation rats without ginkgo. However, duodenal ulcer rats with ginkgo significantly increased erythrocyte superoxide dismutase activity (8.22+/-1.92 U/mg protein versus 4.87+/-1.49 U/mg protein, P<0.05) compared with those without ginkgo. Duodenal ulcer rats without ginkgo significantly increased plasma lipid peroxides (4.18+/-1.12 micromol/mL versus 1.60+/-1.10 micromol/mL and 1.80+/-0.73 micromol/mL, P<0.05) compared with sham operation rats without ginkgo and duodenal ulcer rats with ginkgo during the experimental period.

CONCLUSION

Ginkgo biloba extract can improve weight gain and mucosal healing in duodenal ulcer rats by the actions of cytoprotection and antioxidation.

摘要

目的

研究银杏叶提取物对十二指肠溃疡大鼠细胞保护因子的影响。

方法

将Sprague-Dawley大鼠随机分为四组：无银杏叶假手术组、有银杏叶假手术组、无银杏叶十二指肠溃疡组和有银杏叶十二指肠溃疡组。用500 mL/L乙酸诱导大鼠产生十二指肠溃疡。给有银杏叶组大鼠经尾静脉注射银杏叶提取物，剂量为0.5 mg/(kg/d)，持续7天和14天。

结果

病理结果显示，治疗7天后，有银杏叶的十二指肠溃疡大鼠与无银杏叶的相比，黏膜愈合及炎症情况有所改善。治疗14天后，有银杏叶的十二指肠溃疡大鼠体重增加显著（34.0±4.5 g对24.5±9.5 g，P<0.05），高于无银杏叶的十二指肠溃疡大鼠。与假手术组大鼠相比，十二指肠溃疡大鼠细胞增殖显著增加（有银杏叶和无银杏叶的十二指肠溃疡大鼠中BrdU标记细胞分别为27.4±4.0和27.8±2.3，而有银杏叶和无银杏叶的假手术组大鼠中BrdU标记细胞分别为22.4±3.5和20.8±0.5，P<0.05）。有银杏叶的十二指肠溃疡大鼠黏膜前列腺素E2浓度比无银杏叶的显著增加129%（P<0.05）。与无银杏叶的假手术组大鼠相比，无银杏叶的十二指肠溃疡大鼠十二指肠黏膜和红细胞中超氧化物歧化酶活性显著降低（十二指肠黏膜中分别为19.4±6.7 U/mg蛋白对38.1±18.9 U/mg蛋白，红细胞中分别为4.87±1.49 U/mg蛋白对7.78±2.16 U/mg蛋白，P<0.05）。然而，与无银杏叶的十二指肠溃疡大鼠相比，有银杏叶的十二指肠溃疡大鼠红细胞中超氧化物歧化酶活性显著增加（8.22±1.92 U/mg蛋白对4.87±1.49 U/mg蛋白，P<0.05）。在实验期间，与无银杏叶的假手术组大鼠和有银杏叶的十二指肠溃疡大鼠相比，无银杏叶的十二指肠溃疡大鼠血浆脂质过氧化物显著增加（4.18±1.12 μmol/mL对1.60±1.10 μmol/mL和1.80±0.73 μmol/mL，P<0.05）。