Mustafa Ali, El-Medany Azza, Hagar Hanan H, El-Medany Gamila
Department of Pharmacology, College of Medicine & KKUH, King Saud University, P.O. Box 2925, Riyadh 11461, Saudi Arabia.
Pharmacol Res. 2006 Apr;53(4):324-30. doi: 10.1016/j.phrs.2005.12.010. Epub 2006 Feb 3.
Intestinal inflammatory states, regardless of specific initiating events, share common immunologically mediated pathways of tissue injury and repair. The efficacy of various drugs used to treat ulcerative colitis (UC) was investigated. The aim of the present study is to evaluate the effects of ginkgo biloba extract on the extent and severity of UC caused by intracolonic administration of acetic acid in rats. The inflammatory response was assessed by histology and measurement of myeloperoxidase activity (MPO), reduced glutathione (GSH), tumor necrosis factor (TNF-alpha) and interleukin-1beta (IL-1beta) levels in colon mucosa. Oral pretreatment with Ginkgo biloba in doses of (30, 60, 120 mg kg(-1) body weight) and sulfasalazine in a dose of (500 mg kg(-1) body weight used as reference) for 2 days before induction of colitis and continued for 5 consecutive days, significantly decreased colonic MPO activity, TNF-alpha, and IL-1beta levels and increased GSH concentration. Moreover, Ginkgo biloba attenuated the macroscopic colonic damage and the histopathological changes-induced by acetic acid. These results suggest that Ginkgo biloba may be effective in the treatment of UC through its scavenging effect on oxygen-derived free radicals.
肠道炎症状态，无论具体的起始事件如何，都具有共同的免疫介导的组织损伤和修复途径。研究了用于治疗溃疡性结肠炎（UC）的各种药物的疗效。本研究的目的是评估银杏叶提取物对大鼠结肠内注射乙酸所致UC的程度和严重程度的影响。通过组织学以及测量结肠黏膜中的髓过氧化物酶活性（MPO）、还原型谷胱甘肽（GSH）、肿瘤坏死因子（TNF-α）和白细胞介素-1β（IL-1β）水平来评估炎症反应。在诱导结肠炎前2天，以（30、60、120mg/kg体重）的剂量口服银杏叶提取物，并以（500mg/kg体重作为对照的柳氮磺胺吡啶）连续预处理5天，显著降低了结肠MPO活性、TNF-α和IL-1β水平，并提高了GSH浓度。此外，银杏叶提取物减轻了乙酸诱导的宏观结肠损伤和组织病理学变化。这些结果表明，银杏叶提取物可能通过其对氧衍生自由基的清除作用有效治疗UC。
