Department of Chemistry, Capital Normal University, Beijing 100048, China.
Sensors (Basel). 2012 Dec 13;12(12):17320-9. doi: 10.3390/s121217320.
A sensitive flow injection chemiluminescence assay for carcinoembryonic antigen (CEA) detection based on signal amplification with gold nanoparticles (NPs) is reported in the present work. The sandwich system of CEA/anti-CEA/goat-anti-mouse IgG functionalized Au nanoparticles was used as the sensing platform. In order to improve detection sensitivity, a further gold enlargement step was developed based on the autocatalytic Au deposition of gold nanoprobes via the reduction of AuCl(4)- to Au0 on their surface in the presence of NH(2)OH·HCl. AuCl(4)-, which is a soluble product of gold nanoprobes, served as an analyte in the CL reaction for the indirect measurement of CEA. Under optimized conditions, the CL intensity of the system was linearly related to the logarithm of CEA concentration in the range of 100 pg∙mL-1 to 1,000 ng∙mL-1, with a detection limit of 20 pg∙mL-1.
本文报道了一种基于金纳米粒子（NPs）信号放大的用于癌胚抗原（CEA）检测的灵敏流动注射化学发光分析法。采用 CEA/抗 CEA/功能化金纳米粒子的羊抗鼠 IgG 的三明治体系作为传感平台。为了提高检测灵敏度，在 NH（2）OH·HCl 的存在下，通过将 AuCl（4）-还原为 Au0，在金纳米探针表面上自催化地进行金的放大步骤。AuCl（4）-是金纳米探针的可溶性产物，在 CL 反应中用作分析物，用于间接测量 CEA。在优化条件下，该体系的 CL 强度与 CEA 浓度的对数在 100 pg·mL-1至 1000 ng·mL-1的范围内呈线性关系，检测限为 20 pg·mL-1。
