一种用于PCR鉴定室内空气真菌污染物的快速DNA提取方法。
A rapid DNA extraction method for PCR identification of fungal indoor air contaminants.
作者信息
Dean Timothy R, Betancourt Doris, Menetrez Marc Y
机构信息
National Risk Management Research Laboratory, U.S. Environmental Protection Agency, Research Triangle Park, NC 27711, USA.
出版信息
J Microbiol Methods. 2004 Mar;56(3):431-4. doi: 10.1016/j.mimet.2003.11.015.
PMID:14967235
Abstract
Following air sampling fungal DNA needs to be extracted and purified to a state suitable for laboratory use. Our laboratory has developed a simple method of extraction and purification of fungal DNA appropriate for enzymatic manipulation and Polymerase Chain Reaction (PCR) applications. The methodology described is both rapid and cost effective for use with multiple fungal organisms.
摘要
空气采样后，需要提取并纯化真菌DNA，使其达到适合实验室使用的状态。我们实验室已开发出一种简单的真菌DNA提取和纯化方法，适用于酶促操作和聚合酶链反应（PCR）应用。所描述的方法对于多种真菌生物体来说既快速又经济高效。
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