Gifford D J, Taleisnik E
Botany Department, University of Alberta, Edmonton, Alberta, T6G 2E9, Canada.
Tree Physiol. 1994 Jan;14(1):103-10. doi: 10.1093/treephys/14.1.103.
The effect of a short period of elevated temperature, or heat shock, on protein synthesis was investigated in 2-day-old seedlings of jack pine (Pinus banksiana Lamb.), loblolly pine (P. taeda L.), lodgepole pine (P. contorta Dougl.), black spruce (Picea mariana (Mill.) BSP), and white spruce (P. glauca (Moench) Voss.). In all species, heat shock led to increased [(35)S]methionine incorporation into heat-shock proteins (hsp's) with molecular masses of 83 and 72 kDA. Heat shock also induced synthesis of several low molecular weight proteins that were absent from control seedlings. The low molecular weight hsp's produced by pine seedlings had molecular masses of 27, 24.6, 20.5 and 17.5 kDa, whereas those produced by spruce seedlings had molecular masses of 27.2, 19.8, 18.4, 17.2 and 16.0 kDa. All of the low molecular weight hsp's showed isoelectric variants. Heat shock led to increased [(35)S]methionine incorporation into a group of low molecular weight hsp's that were also present in control seedlings.
研究了短期高温或热激对2日龄的班克松（Pinus banksiana Lamb.）、火炬松（P. taeda L.）、黑松（P. contorta Dougl.）、黑云杉（Picea mariana (Mill.) BSP）和白云杉（P. glauca (Moench) Voss.）幼苗蛋白质合成的影响。在所有物种中，热激导致[³⁵S]甲硫氨酸掺入分子量为83和72 kDa的热激蛋白（hsp's）中增加。热激还诱导了对照幼苗中不存在的几种低分子量蛋白质的合成。松树苗产生的低分子量hsp's分子量为27、24.6、20.5和17.5 kDa，而云杉苗产生的低分子量hsp's分子量为27.2、19.8、18.4、17.2和16.0 kDa。所有低分子量hsp's均表现出等电变体。热激导致[³⁵S]甲硫氨酸掺入对照幼苗中也存在的一组低分子量hsp's中增加。
