沙门氏菌促细胞张力和细胞溶解因子：在中国仓鼠卵巢细胞中的检测及其抗原相关性

Salmonella cytotonic and cytolytic factors: their detection in Chinese hamster ovary cells and antigenic relatedness.

作者信息

Rahman H, Singh V B, Sharma V D, Harne S D

机构信息

Department of Microbiology and Public Health, College of Veterinary Sciences, G.B. Pant University of Agriculture and Technology, Pantnagar, Nainital U.P., India.

出版信息

Vet Microbiol. 1992 Jun 15;31(4):379-87. doi: 10.1016/0378-1135(92)90130-l.

DOI:10.1016/0378-1135(92)90130-l

PMID:1496811

Abstract

Thirty nine strains of Salmonella belonging to 14 different serotypes were screened for the production of cytotonic and cytolytic factors by assayed Chinese hamster ovary (CHO) cells. The cell-free culture supernatants (CFCS) of 32 isolates caused elongation and increase in size of CHO cells while the CFCSs completely lysed the cells. The cytotonic effect in CHO cells correlated precisely with fluid the CFCSs completely lysed the cells. The cytotonic effect in CHO cells correlated precisely with fluid accumulation in the rabbit ligated ileal assay in that 24 isolates yielded positive results in both assays and 13 were found negative in both. Antiserum to S. typhimurium enterotoxin, but not that to cholera toxin or Shiga toxin, neutralized the cytotonic activity present in the CFCS and reacted with the latter in immunodiffusion and coagglutination tests. The cytolytic factor produced by two strains reacted neither with antiserum to Salmonella enterotoxin nor with that the Shiga toxin.

摘要

通过检测中国仓鼠卵巢（CHO）细胞，对属于14种不同血清型的39株沙门氏菌进行了细胞紧张素和细胞溶解因子产生情况的筛选。32株分离株的无细胞培养上清液（CFCS）可导致CHO细胞伸长并增大，而这些CFCS能完全裂解细胞。CHO细胞中的细胞紧张素效应与CFCS完全裂解细胞的情况精确相关。CHO细胞中的细胞紧张素效应与兔结扎回肠试验中的液体蓄积精确相关，因为24株分离株在两种试验中均产生阳性结果，13株在两种试验中均为阴性。鼠伤寒沙门氏菌肠毒素抗血清可中和CFCS中存在的细胞紧张素活性，而霍乱毒素或志贺毒素抗血清则不能，并且在免疫扩散和协同凝集试验中，鼠伤寒沙门氏菌肠毒素抗血清与CFCS发生反应。两株菌产生的细胞溶解因子既不与沙门氏菌肠毒素抗血清反应，也不与志贺毒素抗血清反应。