用于检测沙门氏菌肠毒素的一些生物测定模型和血清学检测方法的比较评估
Comparative evaluation of some bioassay models & serological tests for the detection of Salmonella enterotoxin.
作者信息
Rahman H, Singh V B, Sharma V D, Harne S D
机构信息
Department of Microbiology & Public Health, G.B. Pant University of Agriculture & Technology, Pant Nagar.
出版信息
Indian J Med Res. 1991 Jul;93:217-21.
Two bioassay models viz., rabbit ligated ileal loop (RLIL) and the Chinese hamster ovary (CHO) cell culture and three serological methods viz., enzyme-linked immunosorbent assay (ELISA), Biken and staphylococcal coagglutination (CoA) tests were standardized and used for the detection of Salmonella enterotoxin. A total of 37 strains belonging to 14 different serotypes of Salmonella were tested simultaneously by all the five assay models. Serological tests were standardized with antiserum against the purified enterotoxin of S. typhimurium (P/536). The CHO test was found to be the most sensitive (86.37%) followed by ELISA and CoA test (83.78%) each and Biken test (78.37%). The RLIL test was found to be least sensitive (64.86%) for detection of Salmonella enterotoxin.
建立了两种生物测定模型，即兔结扎回肠袢（RLIL）和中国仓鼠卵巢（CHO）细胞培养模型，以及三种血清学方法，即酶联免疫吸附测定（ELISA）、比肯试验和葡萄球菌凝聚（CoA）试验，并用于检测沙门氏菌肠毒素。使用所有这五种测定模型同时检测了总共37株属于14种不同血清型的沙门氏菌。血清学试验用抗鼠伤寒沙门氏菌（P/536）纯化肠毒素的抗血清进行了标准化。发现CHO试验最敏感（86.37%），其次是ELISA和CoA试验（各83.78%）以及比肯试验（78.37%）。发现RLIL试验在检测沙门氏菌肠毒素方面最不敏感（64.86%）。
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