Sytnik A N
Zh Mikrobiol Epidemiol Immunobiol. 1992 Apr(4):4-6.
The skin autoflora on the pad of a forefinger and the back and palm of a hand was studied in 40 healthy males aged 18-60 years by the modified washing and scraping method of P. Williamson and A. Kligman. 638 cultures of aerobic microorganisms, including coccal (55.3%) and bacilliform (44.7%) microbes, were isolated. In 6 persons (15%) coagulase-positive staphylococci were detected. Out of 10 coagulase negative species of this genus, S. epidermidis, S. saprophyticus and S. warneri occurred most frequently on the skin of hands. The highest density of bacterial populations (10.970 +/- +/- 1.845 cells/sq. cm) was registered on the back of hands, the surface of palms was found to have somewhat lower density (8.679 +/- 1.282 cells/sq. sm) and the skin of forefingers, the lowest density of bacterial populations (6.878 +/- +/- 1.137 cells/sq. sm). 17.5% of examined persons were found to be carriers with S. aureus isolated from their nasal mucosa. S. aureus isolated from the skin surface and the nasal cavity of different persons belonged to different phage variants, but S. aureus isolated from the nasal cavity and the skin of the same person belonged to one phage variant.
采用P. 威廉姆森和A. 克利格曼改良的清洗和刮擦方法，对40名年龄在18至60岁的健康男性食指指腹、手背和手掌的皮肤自生菌丛进行了研究。共分离出638株需氧微生物培养物，其中包括球菌（55.3%）和杆菌（44.7%）。在6人（15%）中检测到凝固酶阳性葡萄球菌。在该属的10种凝固酶阴性菌种中，表皮葡萄球菌、腐生葡萄球菌和沃氏葡萄球菌在手皮肤表面出现的频率最高。手背的细菌种群密度最高（10.970±1.845个/平方厘米），手掌表面的密度略低（8.679±1.282个/平方厘米），食指皮肤的细菌种群密度最低（6.878±1.137个/平方厘米）。17.5%的受检者被发现是从其鼻粘膜中分离出金黄色葡萄球菌的携带者。从不同人皮肤表面和鼻腔分离出的金黄色葡萄球菌属于不同的噬菌体变种，但从同一个人的鼻腔和皮肤分离出的金黄色葡萄球菌属于同一个噬菌体变种。
