Emel'ianova O Iu, Perova N A, Golysheva M A, Berzhets V M
Zh Mikrobiol Epidemiol Immunobiol. 1992 Apr(4):51-4.
To evaluate the antigenic composition and allergenic activity of D. farinae extracts, the methods of rocket and cross immunoelectrophoresis, gel chromatography and the inhibition of the radioallergosorbent test have been used. The presence of 9-20 antigenic components has been established. The fractions of the extract with a molecular weight of 10-200 kD possessed the highest capacity of inhibiting the binding of specific IgE antibodies from the pool of sera from patients sensitized to D. farinae.
为评估粉尘螨提取物的抗原组成和致敏活性,已采用火箭免疫电泳、交叉免疫电泳、凝胶色谱法及放射变应原吸附试验抑制法。已确定存在9 - 20种抗原成分。分子量为10 - 200 kD的提取物组分具有最高的抑制来自对粉尘螨致敏患者血清库中特异性IgE抗体结合的能力。