Le Mao J, Dandeu J P, Rabillon J, Lux M, David B
J Allergy Clin Immunol. 1983 Jun;71(6):588-96. doi: 10.1016/0091-6749(83)90441-4.
Dermatophagoïdes farinae (Df 80d) and Dermatophagoïdes pteronyssinus (Dp 80d) extracts were analyzed for their antigenic and allergenic composition by means of crossed immunoelectrophoresis (CIE) and crossed radioimmunoelectrophoresis (CRIE). By CIE, 11 antigens could be numbered in Df 80d (Df 1 to Df 11) and seven antigens in Dp 80d (Dp 1 to Dp 7). This technique allowed us also to define antigens with common as well as specific parts for the two mite species. Among the antigens of D. farinae and D. pteronyssinus, only the antigen corresponding to Df 5 and Dp 5 seems to bear common epitopes to the two mite species, whereas Df 6 and Dp 4 appear to bear, respectively, specific epitopes of each species. Moreover, Df 11 appears to bear specific epitopes of D. farinae, although it shows a partial identity with Dp 7. By CRIE, on 20 mite-sensitive patients' sera, we identified, for each mite extract, the allergens responsive to human specific IgE. The allergograms show that the majority of mite-sensitive patients react with Df 11 and Df 6 and with Dp 7 and Dp 4. Thus, these antigens can be considered as major allergens. The minor allergens were also identified. None of these antigens was recognized by the control sera. Moreover, we observed that for one antigen (antigen 5) there exist antigenic determinants common to the two species of mites toward the rabbit serum and specific allergenic determinants to the human IgE response. A significant correlation was found between the specific IgE binding in CRIE and in RAST (Spearman coefficient: "rs" = 0.61 p less than 0.01 for Df; "rs" = 0.78 p less than 0.01 for Dp).
采用交叉免疫电泳（CIE）和交叉放射免疫电泳（CRIE）分析了粉尘螨（Df 80d）和屋尘螨（Dp 80d）提取物的抗原和变应原成分。通过CIE，在Df 80d中可编号出11种抗原（Df 1至Df 11），在Dp 80d中可编号出7种抗原（Dp 1至Dp 7）。该技术还使我们能够确定这两种螨类具有共同部分以及特定部分的抗原。在粉尘螨和屋尘螨的抗原中，只有对应于Df 5和Dp 5的抗原似乎对这两种螨类具有共同表位，而Df 6和Dp 4似乎分别具有每种螨类的特异性表位。此外，Df 11似乎具有粉尘螨的特异性表位，尽管它与Dp 7有部分同源性。通过CRIE，在20例对螨敏感患者的血清中，我们针对每种螨提取物鉴定了对人特异性IgE有反应的变应原。变应原图谱显示，大多数对螨敏感的患者对Df 11和Df 6以及Dp 7和Dp 4有反应。因此，这些抗原可被视为主要变应原。也鉴定出了次要变应原。对照血清未识别出这些抗原中的任何一种。此外，我们观察到，对于一种抗原（抗原5），两种螨类对兔血清存在共同的抗原决定簇，而对人IgE反应存在特异性变应原决定簇。在CRIE和RAST中的特异性IgE结合之间发现了显著相关性（斯皮尔曼系数：粉尘螨的“rs” = 0.61，p < 0.01；屋尘螨的“rs” = 0.78，p < 0.01）。
