Estrada Dora Amparo, Quiñoes Martha L, Sierra Diana Maria, Calle David A, Ruiz Fredy, Erazo Holmes F, Linton Yvonne-Marie
Programa de Estudio y Control de Enfermedades Tropicales, PECET, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia.
Biomedica. 2003 Dec;23(4):388-95.
In the Department of Putumayo in southern Colombia, malaria transmission has continued in the absence of the 4 traditional Latin American vector species--Anopheles darlingi, Anopheles nuneztovari, Anopheles albimanus or Anopheles trinkae. Human bait collections yielded Anopheles mosquitoes and a morphological variant of Anopheles benarrochi, the adult females of which can easily be misidentified as Anopheles oswaldoi. Species identification of females of Anopheles in the subgenus Nyssorhynchus is generally difficult due to overlapping morphological characters; therefore, progeny of field collected females were link-reared to assess species identity. Herein a robust method is presented to identify the species Anopheles benarrochi, Anopheles oswaldoi and Anopheles rangeli from southern Colombia, using the morphology of the eggs induced from wild-caught females. Eggs of A. rangeli and A. benarrochi were differentiated on the basis of the anterior crown. In A. rangeli, this feature is positioned apically with high walls. In A. benarrochi, anterior crown is positioned more ventrally with comparatively shorter walls. No crown is present in A. oswaldoi. These differences are clear with the aid of a dissecting microscope and make accurate species determination possible even in field conditions. Egg morphology is shown to be an accurate, albeit indirect, method for the taxonomic determination for the three southern Colombian species and may also be useful in other regions of Latin America where the morphological variant of A. benarrochi is sympatric with A. oswaldoi.
在哥伦比亚南部的普图马约省，尽管缺少4种传统的拉丁美洲病媒物种，即达林按蚊、努涅斯按蚊、白跗按蚊或特林卡按蚊，但疟疾传播仍在继续。人工诱捕采集到了按蚊以及贝纳罗奇按蚊的一个形态变种，其成年雌蚊很容易被误认作奥斯瓦尔多按蚊。由于形态特征重叠，尼氏按蚊亚属雌蚊的物种鉴定通常很困难；因此，对野外采集雌蚊的后代进行关联饲养以评估物种身份。本文介绍了一种可靠的方法，利用野生雌蚊所产卵的形态来鉴定哥伦比亚南部的贝纳罗奇按蚊、奥斯瓦尔多按蚊和兰热利按蚊。兰热利按蚊和贝纳罗奇按蚊的卵可根据前嵴来区分。在兰热利按蚊中，这一特征位于顶端，嵴壁较高。在贝纳罗奇按蚊中，前嵴位置更靠腹侧，嵴壁相对较短。奥斯瓦尔多按蚊没有嵴。借助解剖显微镜，这些差异很明显，即使在野外条件下也能准确鉴定物种。卵形态被证明是一种准确的（尽管是间接的）方法，用于对哥伦比亚南部这三种按蚊进行分类鉴定，在拉丁美洲其他地区可能也有用，因为在这些地区贝纳罗奇按蚊的形态变种与奥斯瓦尔多按蚊同域分布。
