Fajardo Ramos Mayury, González Obando Ranulfo, Fidel Suárez Marco, López David, Wilkerson Richard, Sallum Maria Anice Mureb
Facultad de Ciencias Naturales y Exactas y Facultad de Salud, Universidad del Valle, Cali, Colombia.
Mem Inst Oswaldo Cruz. 2008 Feb;103(1):85-92. doi: 10.1590/s0074-02762008000100013.
Based on the results of comparative analyses of 1,039 specimens of several progenies of Anopheles nuneztovarifrom three localities in Colombia, eight costal wing spot patterns were observed. Patterns I and III were the most frequent: 77.96% and 11.36%, respectively. Using the diagnostic characters ratio of the length of the basal dark area of hind tarsomere II/length of hind tarsomere II, ratio of the length of the humeral pale spot/length of the pre-humeral dark spot, and the ratio of the length of the subcostal pale spot/length of the distal sector dark spot (DS-III2/Ta-III2, HP/PHD, SCP/DSD) approximately 5% of the adult females were misidentified as a species of Nyssorhynchus, different from An. nuneztovari. Approximately 5% of the specimens showed DS-III2/Ta-III2 ratio less than 0.25 (range 0.21 - 0.24), and among them 3.34% shared a HP/PHD ratio less than 1.50. Consequently, 1.52% of An. nuneztovari individuals can be misidentified as Anopheles oswaldoi. In those specimens with the DS-III2/Ta-III2 ratios higher than 0.25, 34.45% displayed SCP/DSD values greater than 0.50 and of these, 3.65% displayed HP/PHD values greater than 1.8. This combination of characters could lead one to misidentify samples of An. nuneztovari as Anopheles rangeli. Similarly, 2.43% of the females could be identified erroneously as either Anopheles aquasalis or Anopheles benarrochi. Individuals with a HP/PHD ratio greater than 2.0, could be misidentified as Anopheles trinkae, Anopheles strodei or Anopheles evansae. A distinct combination of diagnostic characters for An. nuneztovari from Colombia is proposed.
基于对来自哥伦比亚三个地区的努涅斯按蚊几个子代的1039个标本的比较分析结果，观察到八种翅前缘斑点模式。模式I和III最为常见，分别占77.96%和11.36%。使用后跗节II基部深色区域长度/后跗节II长度、肩浅色斑点长度/肩前深色斑点长度以及亚前缘浅色斑点长度/远端扇形深色斑点长度（DS-III2/Ta-III2、HP/PHD、SCP/DSD）的诊断特征比率，约5%的成年雌性被误鉴定为与努涅斯按蚊不同的尼氏按蚊属物种。约5%的标本显示DS-III2/Ta-III2比率小于0.25（范围为0.21 - 0.24），其中3.34%的标本HP/PHD比率小于1.50。因此，1.52%的努涅斯按蚊个体可能被误鉴定为奥斯瓦尔多按蚊。在DS-III2/Ta-III2比率高于0.25的那些标本中，34.45%的标本SCP/DSD值大于0.50，其中3.65%的标本HP/PHD值大于1.8。这种特征组合可能导致将努涅斯按蚊样本误鉴定为兰热按蚊。同样，2.43%的雌性可能被错误鉴定为咸水按蚊或贝氏按蚊。HP/PHD比率大于2.0的个体可能被误鉴定为特氏按蚊、斯特罗德按蚊或埃氏按蚊。提出了哥伦比亚努涅斯按蚊独特的诊断特征组合。
