Bischoff-Ferrari H A, Borchers M, Gudat F, Dürmüller U, Stähelin H B, Dick W
Department of Orthopedic Surgery, University Basel, Basel, Switzerland.
J Bone Miner Res. 2004 Feb;19(2):265-9. doi: 10.1359/jbmr.2004.19.2.265.
Intracellular 1,25-dihydroxyvitamin D receptor (VDR) is expressed in human skeletal muscle tissue. However, it is unknown whether VDR expression in vivo is related to age or vitamin D status, or whether VDR expression differs between skeletal muscle groups.
We investigated these factors and their relation to 1,25-dihydroxyvitamin D receptor (VDR) expression in freshly removed human muscle tissue.
We investigated biopsy specimens of the gluteus medius taken at surgery from 20 female patients undergoing total hip arthroplasty (mean age, 71.6 +/- 14.5; 72% > 65 years) and biopsy specimens of the transversospinalis muscle taken at surgery from 12 female patients with spinal operations (mean age, 55.2 +/- 19.6; 28% > 65 years). The specimens were obtained by immunohistological staining of the VDR using a monoclonal rat antibody to the VDR (Clone no. 9A7). Quantitative VDR expression (number of VDR positive nuclei) was assessed by counting 500 nuclei per specimen and person. Serum concentrations of 25-hydroxyvitamin D and 1,25-dihydroxyvitamin D were assessed at day of admission to surgery.
All muscle biopsy specimens stained positive for VDR. In the univariate analyses, increased age was associated with decreased VDR expression (r = 0.5: p = 0.004), whereas there were no significant correlations between VDR expression and 25-hydroxyvitamin D or 1,25-dihydroxyvitamin D levels. VDR expression did not differ between patients with hip and spinal surgery. In the multivariate analysis, older age was a significant predictor of decreased VDR expression after controlling biopsy location (gluteus medius or the transversospinalis muscle), and 25-hydroxyvitamin D levels (linear regression analysis: beta-estimate = -2.56; p = 0.047).
Intranuclear immunostaining of the VDR was present in muscle biopsy specimens of all orthopedic patients. Older age was significantly associated with decreased VDR expression, independent of biopsy location and serum 25-hydroxyvitamin D levels.
细胞内1,25 - 二羟维生素D受体（VDR）在人体骨骼肌组织中表达。然而，体内VDR表达是否与年龄或维生素D状态相关，或者骨骼肌组之间VDR表达是否存在差异尚不清楚。
我们研究了这些因素及其与新鲜切除的人体肌肉组织中1,25 - 二羟维生素D受体（VDR）表达的关系。
我们研究了20例行全髋关节置换术的女性患者（平均年龄71.6±14.5岁；72%＞65岁）手术中获取的臀中肌活检标本，以及12例行脊柱手术的女性患者（平均年龄55.2±19.6岁；28%＞65岁）手术中获取的横突棘肌活检标本。标本通过使用抗VDR的单克隆大鼠抗体（克隆号9A7）对VDR进行免疫组织化学染色获得。通过对每个标本和个体计数500个细胞核来评估VDR的定量表达（VDR阳性细胞核数量）。在手术入院当天评估血清25 - 羟维生素D和1,25 - 二羟维生素D浓度。
所有肌肉活检标本VDR染色均为阳性。在单因素分析中，年龄增加与VDR表达降低相关（r = 0.5；p = 0.004），而VDR表达与25 - 羟维生素D或1,25 - 二羟维生素D水平之间无显著相关性。髋关节和脊柱手术患者的VDR表达无差异。在多因素分析中，在控制活检部位（臀中肌或横突棘肌）和25 - 羟维生素D水平后，年龄较大是VDR表达降低的显著预测因素（线性回归分析：β估计值 = -2.56；p = 0.047）。
所有骨科患者的肌肉活检标本中均存在VDR的核内免疫染色。年龄较大与VDR表达降低显著相关，与活检部位和血清25 - 羟维生素D水平无关。
