Toyokawa Tatsuya, Yokota Kenji, Mizuno Motowo, Fujinami Yoshihito, Takenaka Ryuta, Okada Hiroyuki, Hayashi Shunji, Hirai Yoshikazu, Oguma Keiji, Shiratori Yasushi
Department of Medicine and Medical Science1 and Department of Bacteriology2, Okayama University Graduate School of Medicine and Dentistry, 2-5-1, Shikata-cho, Okayama 700-8558, Japan 3Division of Bacteriology, Department of Infection and Immunity, Jichi Medical School, Yakushi-ji, Minami, Kawauchi, Kouchi-Gunn, Tochigi 329-0498, Japan.
J Med Microbiol. 2004 Mar;53(Pt 3):207-212. doi: 10.1099/jmm.0.05281-0.
To date, two Helicobacter species, Helicobacter pylori and 'Helicobacter heilmannii' (formerly named 'Gastrospirillum hominis'), have been identified from the human stomach. In this study, we observed non-H. pylori-shaped bacteria in gastric tissue sections and successfully isolated them by cultivation. Elongated bacteria were isolated from a patient with gastric-mucosa-associated lymphoid-tissue lymphoma who had been diagnosed as H. pylori-negative by culture, rapid urease test and histopathology in another hospital. The bacteria were grown only on chocolate agar in a CO2 incubator, appeared more than 10 microm long in histological sections, formed small colonies and showed poor growth in a brain heart infusion broth; these characteristics apparently differed from common clinical isolates of H. pylori. However, the bacteria were identified as H. pylori by PCR of the urease gene, 16S rDNA sequencing, protein profile and antigenicity examined by anti-H. pylori polyclonal antibody. These observations suggest that the H. pylori strain identified in this study may contribute to the development of gastroduodenal diseases in cases judged as H. pylori-negative by ordinary methods.
迄今为止，已从人类胃部鉴定出两种幽门螺杆菌，即幽门螺杆菌和“海尔曼螺杆菌”（原名为“人胃螺菌”）。在本研究中，我们在胃组织切片中观察到非幽门螺杆菌形态的细菌，并通过培养成功分离出它们。从一名胃黏膜相关淋巴组织淋巴瘤患者中分离出细长型细菌，该患者在另一家医院经培养、快速尿素酶试验和组织病理学检查被诊断为幽门螺杆菌阴性。这些细菌仅在二氧化碳培养箱中的巧克力琼脂上生长，在组织切片中长度超过10微米，形成小菌落，并且在脑心浸液肉汤中生长不佳；这些特征明显不同于幽门螺杆菌的常见临床分离株。然而，通过尿素酶基因的PCR、16S rDNA测序、蛋白质谱分析以及用抗幽门螺杆菌多克隆抗体检测抗原性，这些细菌被鉴定为幽门螺杆菌。这些观察结果表明，本研究中鉴定出的幽门螺杆菌菌株可能在通过常规方法判断为幽门螺杆菌阴性的病例中导致胃十二指肠疾病的发生。
