O'Rourke Jani L, Solnick Jay V, Neilan Brett A, Seidel Karin, Hayter Robert, Hansen Lori M, Lee Adrian
School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia, 2052.
Departments of Internal Medicine and Medical Microbiology & Immunology, The Center for Comparative Medicine, University of California, Davis, CA 95616-8645, USA.
Int J Syst Evol Microbiol. 2004 Nov;54(Pt 6):2203-2211. doi: 10.1099/ijs.0.63117-0.
While Helicobacter pylori is accepted as the major bacterial agent of gastric disease in humans, some patients and many animals are infected with a larger, tightly helical-shaped bacterium previously referred to as 'Helicobacter heilmannii' or 'Gastrospirillum hominis'. Taxonomic classification of these bacteria has been hampered by the inability to cultivate them in vitro and by the inadequate discriminatory power of 16S rRNA gene sequence analysis. This study describes the detection and phylogenetic analysis of 26 different gastrospirillum isolates from humans and animals, which incorporates sequence data based on the 16S rRNA and urease genes. Fifteen gastrospirilla detected in humans, primates and pigs clustered with 'Candidatus Helicobacter suis', thus expanding the host range for this organism. By comparison, based on 16S rRNA data, the remaining 11 gastrospirilla could not be differentiated from Helicobacter felis, Helicobacter bizzozeronii and Helicobacter salomonis. However, urease gene sequence analysis allowed for the discrimination of this latter group into four discrete clusters, three of which contained the above recognized species. The fourth cluster contained isolates from human and feline hosts, and should provisionally be considered a unique bacterial species, for which the name 'Candidatus Helicobacter heilmannii' is proposed.
虽然幽门螺杆菌被公认为人类胃部疾病的主要细菌病原体，但一些患者和许多动物感染了一种更大的、紧密螺旋状的细菌，以前被称为“海尔曼螺杆菌”或“人胃螺菌”。这些细菌的分类学分类受到无法在体外培养以及16S rRNA基因序列分析鉴别能力不足的阻碍。本研究描述了从人和动物中分离出的26种不同胃螺菌的检测和系统发育分析，其中纳入了基于16S rRNA和脲酶基因的序列数据。在人类、灵长类动物和猪中检测到的15种胃螺菌与“猪源螺杆菌”聚类，从而扩大了该生物体的宿主范围。相比之下，基于16S rRNA数据，其余11种胃螺菌无法与猫螺杆菌、比氏螺杆菌和所罗门螺杆菌区分开来。然而，脲酶基因序列分析允许将后一组分为四个离散的聚类，其中三个包含上述已识别的物种。第四个聚类包含来自人类和猫科宿主的分离株，应暂时被视为一个独特的细菌物种，为此提出了“海尔曼螺杆菌”的名称。
