Meister Gunter, Landthaler Markus, Dorsett Yair, Tuschl Thomas
Laboratory of RNA Molecular Biology, The Rockefeller University, New York, New York 10021, USA.
RNA. 2004 Mar;10(3):544-50. doi: 10.1261/rna.5235104.
A large number of miRNAs have recently been discovered in plants and animals. Development of reverse genetic approaches that act to inhibit microRNA function would facilitate the study of this new class of noncoding RNA. Here we show that 2'-O-methyl oligoribonucleotides, but not 2'-deoxyoligonucleotides specifically inactivate the RNAi activity associated with miRNA-protein complexes in human cell extracts as well as in cultured human cells.
最近在植物和动物中发现了大量的微小RNA（miRNA）。开发能够抑制微小RNA功能的反向遗传学方法将有助于对这类新型非编码RNA的研究。在此我们表明，2'-O-甲基寡核糖核苷酸而非2'-脱氧寡核苷酸能够特异性地使人类细胞提取物以及培养的人类细胞中与miRNA-蛋白质复合物相关的RNA干扰（RNAi）活性失活。
