Van Poucke M, Yerle M, Chardon P, Jacobs K, Genêt C, Mattheeuws M, Van Zeveren A, Peelman L J
Department of Animal Nutrition, Genetics, Breeding and Ethology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
Cytogenet Genome Res. 2003;102(1-4):133-8. doi: 10.1159/000075738.
We report here the localisation of BAIAP1 (13q24), HTR1F (13q45), PTPRG (13q23) and UBE1C (13q24) by fluorescence in situ hybridisation (FISH), and BAIAP1 (Swr2114; 21 cR; LOD = 11.03), GATA2 (Sw2448; 37 cR; LOD = 8.26), IL5RA (Swr2114; 64 cR; LOD = 3.85), LMCD1 (Sw2450; 61 cR; LOD = 4.73), MME (CP; 50 cR; LOD = 7.75), RYK (Swc22; 12 cR; LOD = 18.62) and SGU003 (Sw1876; 6 cR; LOD = 16.99) by radiation hybrid (RH) mapping to porcine chromosome 13 (SSC13). The mapping of these 10 different loci (all mapped to human chromosome 3; HSA3) not only confirms the extended conservation of synteny between HSA3 and SSC13, but also defines more precisely the regions with conserved linkage. The syntenic region of the centromeric part of SSC13 was determined by isolating porcine bacterial artificial chromosome (BAC) clones (842D4 and 1031H1) using primers amplifying porcine microsatellite markers S0219 and S0076 (mapped to this region). Sequence comparison of the BAC end sequences with the human genome sequence showed that the centromeric part of SSC13 is homologous with HSA3p24.
我们在此报告通过荧光原位杂交（FISH）对BAIAP1（13q24）、HTR1F（13q45）、PTPRG（13q23）和UBE1C（13q24）进行的定位，以及通过辐射杂种（RH）定位将BAIAP1（Swr2114；21厘摩；LOD = 11.03）、GATA2（Sw2448；37厘摩；LOD = 8.26）、IL5RA（Swr2114；64厘摩；LOD = 3.85）、LMCD1（Sw2450；61厘摩；LOD = 4.73）、MME（CP；50厘摩；LOD = 7.75）、RYK（Swc22；12厘摩；LOD = 18.62）和SGU003（Sw1876；6厘摩；LOD = 16.99）定位到猪13号染色体（SSC13）上。这10个不同基因座（均映射到人类3号染色体；HSA3）的定位不仅证实了HSA3和SSC13之间同源性的广泛保守性，还更精确地定义了具有保守连锁的区域。通过使用扩增猪微卫星标记S0219和S0076（映射到该区域）的引物分离猪细菌人工染色体（BAC）克隆（842D4和1031H1），确定了SSC13着丝粒部分的同源区域。将BAC末端序列与人类基因组序列进行比较表明，SSC13的着丝粒部分与HSA3p24同源。
