Van Poucke M, Yerle M, Tuggle C, Piumi F, Genêt C, Van Zeveren A, Peelman L J
Department of Animal Nutrition, Genetics, Breeding and Ethology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
Cytogenet Cell Genet. 2001;93(3-4):297-303. doi: 10.1159/000057001.
In order to expand the comparative map between human chromosome 3 (HSA3) and porcine chromosome 13 (SSC13), seven genes from HSA3 were mapped on SSC13 by fluorescence in situ hybridisation (FISH), viz. ACAA1, ACPP, B4GALT4, LTF, MYLK, PDHB and RARB. With a view to integrating this expanded comparative map with the existing SSC13 linkage map, we used the INRA-University of Minnesota porcine Radiation Hybrid panel (IMpRH) to localize more precisely and to order 15 genes on the SSC13 map, viz. ACPP, ADCY5, APOD, BCHE, CD86, DRD3, GAP43, PCCB, RAF1, RHO, SI, TF, TFRC, TOP2B and ZNF148. In this way, we were able to create an integrated map, containing 38 type I and 81 type II markers, by correlating the linkage, radiation hybrid (RH) and cytogenetic maps of SSC13. This integrated map will give us the opportunity to take maximal advantage of the comparative mapping strategy for positional candidate cloning of genes responsible for economically important traits.
为了扩展人类3号染色体（HSA3）与猪13号染色体（SSC13）之间的比较图谱，通过荧光原位杂交（FISH）将来自HSA3的7个基因定位到SSC13上，即ACAA1、ACPP、B4GALT4、LTF、MYLK、PDHB和RARB。为了将这个扩展的比较图谱与现有的SSC13连锁图谱整合起来，我们使用了法国国家农业研究院-明尼苏达大学猪辐射杂种细胞系板（IMpRH），以便更精确地定位并排列SSC13图谱上的15个基因，即ACPP、ADCY5,、APOD、BCHE、CD86、DRD3、GAP43、PCCB,、RAF1、RHO、SI、TF、TFRC、TOP2B和ZNF148。通过这种方式，我们通过关联SSC13的连锁、辐射杂种（RH）和细胞遗传图谱，创建了一个包含38个I型和81个II型标记的整合图谱。这个整合图谱将使我们有机会充分利用比较图谱策略，对负责经济重要性状的基因进行定位候选克隆。
