整合鸡基因组资源以实现全基因组测序。

Integration of chicken genomic resources to enable whole-genome sequencing.

作者信息

Aerts J, Crooijmans R, Cornelissen S, Hemmatian K, Veenendaal T, Jaadar A, van der Poel J, Fillon V, Vignal A, Groenen M

机构信息

Animal Breeding and Genetics Group, Wageningen University, Wageningen, The Netherlands.

出版信息

Cytogenet Genome Res. 2003;102(1-4):297-303. doi: 10.1159/000075766.

DOI:10.1159/000075766

PMID:14970720

Abstract

Different genomic resources in chicken were integrated through the Wageningen chicken BAC library. First, a BAC anchor map was created by screening this library with two sets of markers: microsatellite markers from the consensus linkage map and markers created from BAC end sequencing in chromosome walking experiments. Second, HINdIII digestion fingerprints were created for all BACs of the Wageningen chicken BAC library. Third, cytogenetic positions of BACs were assigned by FISH. These integrated resources will facilitate further chromosome-walking experiments and whole-genome sequencing.

摘要

通过瓦赫宁根鸡细菌人工染色体（BAC）文库整合了鸡的不同基因组资源。首先，通过用两组标记筛选该文库创建了一个BAC锚定图谱：来自一致性连锁图谱的微卫星标记以及在染色体步移实验中从BAC末端测序产生的标记。其次，为瓦赫宁根鸡BAC文库的所有BAC创建了HINdIII酶切指纹图谱。第三，通过荧光原位杂交（FISH）确定了BAC的细胞遗传学位置。这些整合的资源将有助于进一步的染色体步移实验和全基因组测序。