Rabie Tarik S K M, Crooijmans Richard P M A, Morisson Mireille, Andryszkiewicz Joanna, van der Poel Jan J, Vignal Alain, Groenen Martien A M
Wageningen Institute of Animal Sciences, Animal Breeding and Genetics Group, Wageningen University, Marijkeweg 40, 6709 PG Wageningen, The Netherlands.
Mamm Genome. 2004 Jul;15(7):560-9. doi: 10.1007/s00335-004-2362-8.
The mapping resolution of the physical map for chicken Chromosome 4 (GGA4) was improved by a combination of radiation hybrid (RH) mapping and bacterial artificial chromosome (BAC) mapping. The ChickRH6 hybrid panel was used to construct an RH map of GGA4. Eleven microsatellites known to be located on GGA4 were included as anchors to the genetic linkage map for this chromosome. Based on the known conserved synteny between GGA4 and human Chromosomes 4 and X, sequences were identified for the orthologous chicken genes from these human chromosomes by BLAST analysis. These sequences were subsequently used for the development of STS markers to be typed on the RH panel. Using a logarithm of the odds (LOD) threshold of 5.0, nine linkage groups could be constructed which were aligned with the genetic linkage map of this chromosome. The resulting RH map consisted of the 11 microsatellite markers and 50 genes. To further increase the number of genes on the map and to provide additional anchor points for the physical BAC map of this chromosome, BAC clones were identified for 22 microsatellites and 99 genes. The combined RH and BAC mapping approach resulted in the mapping of 61 genes on GGA4 increasing the resolution of the chicken-human comparative map for this chromosome. This enhanced comparative mapping resolution enabled the identification of multiple rearrangements between GGA4 and human Chromosomes 4q and Xp.
通过辐射杂种（RH）作图和细菌人工染色体（BAC）作图相结合的方法，提高了鸡4号染色体（GGA4）物理图谱的作图分辨率。使用ChickRH6杂种细胞系构建了GGA4的RH图谱。已知位于GGA4上的11个微卫星被用作该染色体遗传连锁图谱的锚定标记。基于GGA4与人类4号和X号染色体之间已知的保守同线性，通过BLAST分析从这些人类染色体中鉴定出鸡直系同源基因的序列。这些序列随后用于开发要在RH细胞系上分型的STS标记。使用5.0的优势对数（LOD）阈值，可以构建9个连锁群，这些连锁群与该染色体的遗传连锁图谱对齐。所得的RH图谱由11个微卫星标记和50个基因组成。为了进一步增加图谱上的基因数量，并为该染色体的物理BAC图谱提供额外的锚定标记，鉴定了22个微卫星和99个基因的BAC克隆。RH和BAC作图相结合的方法导致在GGA4上定位了61个基因，提高了该染色体鸡-人比较图谱的分辨率。这种提高的比较作图分辨率使得能够鉴定GGA4与人类4号染色体长臂（4q）和X染色体短臂（Xp）之间的多个重排。
