Reed K M, Chaves L D, Garbe J R, Da Y, Harry D E
Animal Biotechnology Center, University of Minnesota, St Paul, MN 55108, USA.
Cytogenet Genome Res. 2003;102(1-4):331-9. doi: 10.1159/000075771.
Efforts to build a comprehensive genetic linkage map for the turkey (Meleagris gallopavo) have focused on development of genetic markers and experimental resource families. In this study, PCR amplification was attempted for 772 microsatellite markers that had been previously developed for three avian species (chicken, quail and turkey). Allelic polymorphism at 410 markers (53.1% of total examined) was determined by genotyping ten individuals (six F1 parents and four grandparents) in a new resource population specifically developed for genetic linkage mapping. Of these 410 markers, 109 (26.6%) were polymorphic in the tested individuals, with an average of 2.3 alleles per marker. Higher levels of polymorphism were found for the turkey-specific markers (61.1%) than for the chicken (22.7%) or quail-specific markers (33.3%). To test the fidelity of the matings, demonstrate the power of these families for linkage analysis, and determine genetic linkage relationships, 86 polymorphic markers were genotyped for up to 224 birds including founder grandparents, parents and F2 progeny. Linkage relationships for many of the chicken markers elucidated in the turkey were comparable to those observed in the chicken. These data demonstrate that the new UMN/NTBF resource population will provide a solid foundation for constructing a comparative genetic map of the turkey.
构建火鸡（吐绶鸡）综合遗传连锁图谱的工作主要集中在遗传标记的开发和实验资源家系的建立上。在本研究中，对先前为三种禽类（鸡、鹌鹑和火鸡）开发的772个微卫星标记进行了PCR扩增。通过对专门为遗传连锁图谱构建而培育的一个新资源群体中的10个个体（6个F1代亲本和4个祖父母）进行基因分型，确定了410个标记（占总检测数的53.1%）的等位基因多态性。在这410个标记中，有109个（26.6%）在测试个体中表现出多态性，每个标记平均有2.3个等位基因。发现火鸡特异性标记的多态性水平（61.1%）高于鸡特异性标记（22.7%）或鹌鹑特异性标记（33.3%）。为了检验交配的准确性，证明这些家系用于连锁分析的能力，并确定遗传连锁关系，对包括创始祖父母、亲本和F2代后代在内的多达224只鸟的86个多态性标记进行了基因分型。在火鸡中阐明的许多鸡标记的连锁关系与在鸡中观察到的相当。这些数据表明，新的UMN/NTBF资源群体将为构建火鸡的比较遗传图谱提供坚实的基础。
