Rexroad C E, Lee Y, Keele J W, Karamycheva S, Brown G, Koop B, Gahr S A, Palti Y, Quackenbush J
USDA/ARS National Center for Cool and Cold Water Aquaculture, Kearneysville, West Virginia 25430, USA.
Cytogenet Genome Res. 2003;102(1-4):347-54. doi: 10.1159/000075773.
Expressed sequence tag (EST) projects have produced extremely valuable resources for identifying genes affecting phenotypes of interest. A large-scale EST sequencing project for rainbow trout was initiated to identify and functionally annotate as many unique transcripts as possible. Over 45,000 5' ESTs were obtained by sequencing clones from a single normalized library constructed using mRNA from six tissues. The production of this sequence data and creation of a rainbow trout Gene Index eliminating redundancy and providing annotation for these sequences will facilitate research in this species.
表达序列标签（EST）项目为鉴定影响目标表型的基因提供了极其宝贵的资源。为了尽可能多地鉴定和功能注释独特转录本，启动了一项虹鳟鱼大规模EST测序项目。通过对来自使用六个组织的mRNA构建的单个标准化文库的克隆进行测序，获得了超过45,000个5' EST。这些序列数据的产生以及虹鳟鱼基因索引的创建，消除了冗余并为这些序列提供了注释，将有助于该物种的研究。
