Passey R B, Maluf K C
Department of Pathology, University of Oklahoma, Oklahoma City 73034.
Arch Pathol Lab Med. 1992 Jul;116(7):757-60.
This article discusses the relationship of calibration and linearity using the example of glutaraldehyde assayed by high-performance liquid chromatography. Assuming adequate precision and a lack of interference, the relationship of calibration to a method's linear range becomes all important to achieving quality analytical results. Linearity is an objective description of the relationship between a quantitative method's final answer and true analyte concentration. Calibration brings this relationship into correspondence with calibrator concentration. The reportable range is that range within which a specified method produces results within defined allowable error without resorting to dilution of the specimen. The reportable range assumes a linear relationship between true analyte concentration and analytical results.
本文以高效液相色谱法测定戊二醛为例，讨论校准与线性的关系。假设具有足够的精密度且不存在干扰，校准与方法线性范围的关系对于获得高质量分析结果至关重要。线性是定量方法的最终答案与真实分析物浓度之间关系的客观描述。校准使这种关系与校准物浓度相对应。可报告范围是指特定方法在不稀释样本的情况下，在规定的允许误差范围内产生结果的范围。可报告范围假定真实分析物浓度与分析结果之间存在线性关系。
