用于日立系列化学分析仪的酶法同型半胱氨酸检测方法的评估
Evaluation of an enzymatic homocysteine assay for the Hitachi series chemistry analyzer.
作者信息
Kellogg Mark D, Parker Reina, Ricupero Anthony, Rifai Nader
机构信息
Department of Laboratory Medicine, Children's Hospital, Harvard Medical School, 300 Longwood Avenue, Boston, MA 02155, USA.
出版信息
Clin Chim Acta. 2005 Apr;354(1-2):117-22. doi: 10.1016/j.cccn.2004.11.028. Epub 2005 Jan 15.
BACKGROUND
Homocysteine is an essential amino acid, whose clinical utilization includes detection of homocystinuria, vitamin B12 and folate deficiencies and cardiovascular disease management. We report assay performance of the Catch reagents on the Hitachi 917 chemistry analyzer.
METHODS
Linearity, recovery, imprecision and interference from lipids, bilirubin, hemoglobin and ascorbic acid were determined on a Hitachi 917 chemistry analyzer. Split sample aliquots were assayed using the described method, by HPLC and fluorescence polarization immunoassay for method comparisons.
RESULTS
The assay was linear to at least 45 micromol/l. Intra- and interassay variation ranged from 1.7% to 3.4% and 3.3% to 6.7%, respectively. Interference was observed when hemoglobin concentrations was >7 g/l and intralipid >5 g/l. No interference was observed from bilirubin or ascorbic acid. The assay was in good agreement with values obtained by HPLC and fluorescence polarization immunoassay.
CONCLUSIONS
The performance of the Catch homocysteine assay was acceptable on the Hitachi 917 chemistry analyzer.
背景
同型半胱氨酸是一种必需氨基酸，其临床应用包括检测同型胱氨酸尿症、维生素B12和叶酸缺乏以及心血管疾病管理。我们报告了日立917化学分析仪上Catch试剂的检测性能。
方法
在日立917化学分析仪上测定线性、回收率、不精密度以及来自脂质、胆红素、血红蛋白和抗坏血酸的干扰。使用所述方法对分离的样品等分试样进行检测，通过高效液相色谱法（HPLC）和荧光偏振免疫分析法进行方法比较。
结果
该检测方法在至少45微摩尔/升的范围内呈线性。批内和批间变异分别为1.7%至3.4%和3.3%至6.7%。当血红蛋白浓度>7克/升和脂类>5克/升时观察到干扰。未观察到胆红素或抗坏血酸的干扰。该检测方法与通过HPLC和荧光偏振免疫分析法获得的值高度一致。
结论
在日立917化学分析仪上，Catch同型半胱氨酸检测方法的性能是可接受的。
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