Tomida Mihoko, Ishimaru Jun-Ichi, Hayashi Tomoya, Nakamura Koji, Murayama Koichi, Era Seiichi
Department of Physiology, Tokyo Women's Medical University, Shinjuku-ku, Tokyo, 162-8666 Japan.
Jpn J Physiol. 2003 Oct;53(5):351-5. doi: 10.2170/jjphysiol.53.351.
The redox states of albumin in serum and synovial fluid (SF) were analyzed in 35 female patients with temporomandibular joint disorders and 9 asymptomatic female healthy volunteers by high-performance liquid chromatographic (HPLC) analysis. Human serum albumin (HSA) is divided by HPLC analysis into three fractions: mercaptalbumin (HMA, reduced form) and nonmercaptalbumin (HNA-1 and HNA-2, oxidized forms). It was found that the characterized values of albumin in SF of patients with the disorders were the fraction of HMA (f (HMA), 65.8 +/- 9.1%), the fraction of HNA-1 (f (HNA-1), 31.3 +/- 8.8%), and the fraction of HNA-2 (f (HNA-2), 2.9 +/- 0.9%), respectively, and similarly the values of albumin in serum of same subjects were f (HMA), 79.4 +/- 4.0%; f (HNA-1), 18.7 +/- 3.8%; and f (HNA-2), 1.9 +/- 0.5%. The HNA fractions in SF were significantly higher than those in serum (p < 0.0001). The SF of patients and even control showed more oxidative condition compared with sera of the same subjects. Each fraction of SF showed significant difference in control (p < 0.05), and greater significance in patients (p < 0.0001). Furthermore, the HNA-2 fraction (f (HNA-2), 2.9 +/- 0.9%) in SF of the patients with temporomandibular joint disorders revealed a significantly higher value (p < 0.01) than that (f (HNA-2), 1.7 +/- 0.3%) of controls. These findings suggested that SF of the temporomandibular joint is more locally oxidized than serum; thus oxidative factors are concerned in the pathogenesis of the temporomandibular joint disorders. It was also suggested that synovial albumin may play a scavenging role against the intraarticular oxidative stress.
通过高效液相色谱（HPLC）分析，对35名患有颞下颌关节紊乱症的女性患者和9名无症状女性健康志愿者的血清及滑液（SF）中白蛋白的氧化还原状态进行了分析。通过HPLC分析，人血清白蛋白（HSA）可分为三个组分：巯基白蛋白（HMA，还原形式）和非巯基白蛋白（HNA - 1和HNA - 2，氧化形式）。结果发现，患有颞下颌关节紊乱症患者的滑液中白蛋白的特征值分别为HMA组分（f（HMA），65.8±9.1%）、HNA - 1组分（f（HNA - 1），31.3±8.8%）和HNA - 2组分（f（HNA - 2），2.9±0.9%），同样，同一受试者血清中白蛋白的值分别为f（HMA），79.4±4.0%；f（HNA - 1），18.7±3.8%；f（HNA - 2），1.9±0.5%。滑液中的HNA组分显著高于血清中的HNA组分（p < 0.0001）。与同一受试者的血清相比，患者甚至对照组的滑液显示出更多的氧化状态。滑液的每个组分在对照组中显示出显著差异（p < 0.05），在患者组中差异更显著（p < 0.0001）。此外，患有颞下颌关节紊乱症患者滑液中的HNA - 2组分（f（HNA - 2），2.9±0.9%）显著高于对照组（f（HNA - 2），1.7±0.3%）（p < 0.01）。这些发现表明，颞下颌关节的滑液比血清在局部更易氧化；因此，氧化因素与颞下颌关节紊乱症的发病机制有关。还表明滑膜白蛋白可能对关节内的氧化应激起清除作用。
