Suzuki E, Yasuda K, Takeda N, Sakata S, Era S, Kuwata K, Sogami M, Miura K
Third Department of Internal Medicine, Gifu University, School of Medicine, Japan.
Diabetes Res Clin Pract. 1992 Dec;18(3):153-8. doi: 10.1016/0168-8227(92)90140-m.
High-performance liquid chromatographic (HPLC) analysis of human serum albumin (HSA) on Asahipak GS-520H columns at neutral pH (6.87) showed a clear resolution of human mercaptalbumin (HMA) and nonmercaptalbumin (HNA), which are reduced and oxidized form of HSA, respectively. We studied the conversion of HMA to HNA (mercapt-nonmercapt conversion) as an index of oxidative change of the tissues and organs in 28 normal subjects and in a total of 47 patients with non-insulin dependent diabetes mellitus (NIDDM). Mean (+/- SD) values of the HMA fraction of HSA, f(HMA), [HMA/(HMA + HNA)], was significantly lower in NIDDM patients than in normal subjects (0.63 +/- 0.067 vs 0.75 +/- 0.028, P < 0.001). It was lower in poorly controlled NIDDM patients (0.63 +/- 0.058, n = 20) than in well controlled NIDDM patients (0.67 +/- 0.032, n = 9) (P < 0.05). Plasma glucose values sampled on occasions including overnight fasting and postprandial ones (r = -0.441, n = 47, P < 0.01), but not plasma glucose values sampled on overnight fasting (r = -0.345, n = 29) or postprandial (r = -0.467, n = 18) conditions and HbA1c (r = -0.211, n = 34), negatively correlated with the f(HMA) values, indicating that mercapt-nonmercapt conversion may not be due to cumulative hyperglycemia over a month, but due to short-term alteration in blood glucose level. The presence or absence of diabetic complications including nephropathy, retinopathy and neuropathy did not affect the f(HMA) values. In conclusion, decreased f(HMA) values in the diabetic patients suggested the presence of a rapidly altered oxidative change of albumin due to hyperglycemia.
在中性pH值（6.87）条件下，使用旭化成pak GS - 520H柱对人血清白蛋白（HSA）进行高效液相色谱（HPLC）分析，结果显示人巯基白蛋白（HMA）和非巯基白蛋白（HNA）得到了清晰的分离，它们分别是HSA的还原形式和氧化形式。我们研究了28名正常受试者以及总共47名非胰岛素依赖型糖尿病（NIDDM）患者中HMA向HNA的转化（巯基 - 非巯基转化），以此作为组织和器官氧化变化的指标。NIDDM患者中HSA的HMA组分占比，即f(HMA)，[HMA/(HMA + HNA)]的均值（±标准差）显著低于正常受试者（0.63±0.067对0.75±0.028，P < 0.001）。血糖控制不佳的NIDDM患者（0.63±0.058，n = 20）的该值低于血糖控制良好的NIDDM患者（0.67±0.032，n = 9）（P < 0.05）。包括空腹过夜和餐后血糖值在内的多次采样的血浆葡萄糖值（r = -0.441，n = 47，P < 0.01），但空腹过夜（r = -0.345，n = 29）或餐后（r = -0.467，n = 18）血糖值以及糖化血红蛋白（HbA1c）（r = -0.211，n = 34）与f(HMA)值呈负相关，这表明巯基 - 非巯基转化可能并非由于一个月内累积的高血糖，而是由于血糖水平的短期变化。是否存在包括肾病、视网膜病变和神经病变在内的糖尿病并发症并不影响f(HMA)值。总之，糖尿病患者中f(HMA)值降低表明存在因高血糖导致的白蛋白快速氧化变化。
