Era S, Kuwata K, Imai H, Nakamura K, Hayashi T, Sogami M
Department of Physiology, Gifu University School of Medicine, Japan.
Biochim Biophys Acta. 1995 Feb 22;1247(1):12-6. doi: 10.1016/0167-4838(94)00166-e.
Human serum albumin (HSA) is the mixture of human mercaptalbumin (HMA, reduced form) and human nonmercaptalbumin (HNA, oxidized form). We developed a rapid and concise HPLC system to obtain the clear resolution of HSA into HMA and HNA, using an Asahipak GS-520H column. The mean value of the fraction of HMA (f(HMA)) for healthy young male subjects was 0.76 +/- 0.04 (n = 54). However, the f(HMA, 60-90) value for healthy elderly subjects (where the numbers in brackets indicate the range of ages) was 0.48 +/- 0.06 (n = 183). In healthy elderly subjects, f(HMA) was significantly lower than in healthy young male subjects, indicating that HSA in the elderly becomes more oxidized than in the young subjects. Consequently, we suggest that one of the important functions of serum albumin could be to participate in the maintenance of a constant redox potential in the extracellular fluids, thus securing a certain redox buffer capacity. f(HMA) on HSA might reflect this redox buffer capacity with age.
人血清白蛋白（HSA）是人体巯基白蛋白（HMA，还原形式）和人体非巯基白蛋白（HNA，氧化形式）的混合物。我们开发了一种快速简便的高效液相色谱系统，使用Asahipak GS-520H柱将HSA清晰分离为HMA和HNA。健康年轻男性受试者的HMA分数（f(HMA)）平均值为0.76±0.04（n = 54）。然而，健康老年受试者（括号中的数字表示年龄范围）的f(HMA, 60 - 90)值为0.48±0.06（n = 183）。在健康老年受试者中，f(HMA)显著低于健康年轻男性受试者，这表明老年人的HSA比年轻人的HSA氧化程度更高。因此，我们认为血清白蛋白的重要功能之一可能是参与维持细胞外液中恒定的氧化还原电位，从而确保一定的氧化还原缓冲能力。HSA上的f(HMA)可能随年龄反映这种氧化还原缓冲能力。
