Wang Shu-zhen, Sun Jian-heng, Zhang Wei, Jin Shun-qian, Wang Hong-ping, Jin Yu-sheng, Qu Ping, Liu Yi, Li Mo
Department of Obstetrics and Gynecology, Chaoyang Hospital, Capital University of Medical Sciences, Beijing 100020, China.
Chin Med J (Engl). 2004 Feb;117(2):202-6.
It was reported that telomerase expression is closely associated with cellular immortality and cancer. This study was designed to investigate the relationship between telomerase expression and the carcinogenesis of cervical cancer, the possible use of telomerase as a marker of cervical intraepithelial neoplasia (CIN) progression or regression, and the natural history of CIN.
Telomeric repeat amplification protocol (TRAP) assay was used to measure telomerase activity in cervical scrapings and biopsy samples obtained from 105 cases affected with various cervical conditions, including chronic cervicitis (n = 20), CIN (n = 64, 16 cases of CIN I, 20 cases of CIN II, and 28 cases of CIN III), and invasive squamous cell carcinoma (n = 21).
In exfoliated cell samples, telomerase activity was detected in 5 of 20 (25.0%) cases of cervicitis, 10 of 16 (62.5%) cases of CIN I, 11 of 20 (55.0%) cases of CIN II, 23 of 28 (82.1%) cases of CIN III, and 13 of 21 (61.9%) cases of carcinoma. In cervical biopsy samples, telomerase activity was detected in 6 of 20 (30.0%) cases of cervicitis, 8 of 16 (50.0%) cases of CIN I, 9 of 20 (45.0%) cases of CIN II, 27 of 28 (96.4%) cases of CIN III, and 20 of 21 (95.2%) cases of carcinoma. Telomerase activation was significantly higher in CIN samples than in cervicitis samples. Telomerase activity was detected at similar frequency in samples from cervical scrapings and cervical biopsies.
These results seem to suggest that telomerase expression may be associated with carcinogenesis of the cervix. TRAP assay of cervical scraping samples could be used to monitor and predict the development of CIN in clinical practice.
据报道，端粒酶表达与细胞永生化及癌症密切相关。本研究旨在探讨端粒酶表达与宫颈癌发生的关系、端粒酶作为宫颈上皮内瘤变（CIN）进展或消退标志物的可能用途以及CIN的自然病程。
采用端粒重复序列扩增法（TRAP）检测从105例患有各种宫颈疾病的患者获取的宫颈刮片和活检样本中的端粒酶活性，这些疾病包括慢性宫颈炎（n = 20）、CIN（n = 64，其中CIN I 16例、CIN II 20例、CIN III 28例）和浸润性鳞状细胞癌（n = 21）。
在脱落细胞样本中，20例宫颈炎患者中有5例（25.0%）检测到端粒酶活性，16例CIN I患者中有10例（62.5%），20例CIN II患者中有11例（55.0%），28例CIN III患者中有23例（82.1%），21例癌患者中有13例（61.9%）。在宫颈活检样本中，20例宫颈炎患者中有6例（30.0%）检测到端粒酶活性，16例CIN I患者中有8例（50.0%），20例CIN II患者中有9例（45.0%），28例CIN III患者中有27例（96.4%），21例癌患者中有20例（95.2%）。CIN样本中端粒酶激活显著高于宫颈炎样本。宫颈刮片和宫颈活检样本中端粒酶活性检测频率相似。
这些结果似乎表明端粒酶表达可能与宫颈癌发生有关。宫颈刮片样本的TRAP检测可用于临床实践中监测和预测CIN的发展。
