Morelva Toro de Méndez, Antonio Llombart Bosch
Exfoliative Cytology, Faculty of Pharmaceutics and Analytical Biosciences, University of Los Andes, Mérida, Venezuela.
Virchows Arch. 2009 Sep;455(3):235-43. doi: 10.1007/s00428-009-0818-7. Epub 2009 Aug 14.
Ubiquitin and telomerase immunohistochemical expression patterns in cervical cancer were compared with normal cervical tissue samples. Eighty-one cervical cancer cases and 22 normal exo-endocervical tissue were examined with polyclonal antibody for ubiquitin and 44G12 clone for telomerase using tissue microarrays. The results were interpreted using a semiquantitative scale The average age of patients was 50.67 years. The most frequent histological types were moderately differentiated epidermoid carcinoma (43.5%), according to the degree of differentiation, and endocervical adenocarcinoma (42.1%). Immunohistochemical findings were as follows: 98.7% of cervical cancers showed immunoexpression for ubiquitin and 52.6% for telomerase. Statistically significant differences were found in tumor immunoreactivity when compared with control tissue (p < 0.0007) for both biomarkers. There was no significant difference in biomarker expression at different histological types of tumors, although telomerase was less expressed in endocervical adenocarcinoma. Our findings confirm that abnormal immunoexpression pattern of ubiquitin and telomerase is common in HPV-positive cervical cancer, indicating the existence of an intense degradation of proteins, subsequent cellular immortalization and maintenance of the malignant phenotype.
将宫颈癌中泛素和端粒酶的免疫组化表达模式与正常宫颈组织样本进行比较。使用组织微阵列，用泛素多克隆抗体和端粒酶44G12克隆对81例宫颈癌病例和22例正常宫颈内外膜组织进行检测。结果采用半定量量表进行解读。患者的平均年龄为50.67岁。根据分化程度，最常见的组织学类型为中分化鳞状细胞癌（43.5%）和宫颈内膜腺癌（42.1%）。免疫组化结果如下：98.7%的宫颈癌显示泛素免疫表达，52.6%显示端粒酶免疫表达。与对照组织相比，两种生物标志物在肿瘤免疫反应性方面均存在统计学显著差异（p < 0.0007）。不同组织学类型的肿瘤在生物标志物表达上无显著差异，尽管宫颈内膜腺癌中端粒酶表达较少。我们的研究结果证实，泛素和端粒酶的异常免疫表达模式在人乳头瘤病毒阳性宫颈癌中很常见，表明存在强烈的蛋白质降解、随后的细胞永生化以及恶性表型的维持。
