Cavalieri Elisabetta, Mariotto Sofia, Fabrizi Cinzia, de Prati Alessandra Carcereri, Gottardo Rossella, Leone Stefano, Berra Luigi Valentino, Lauro Giuliana Maria, Ciampa Anna Rosa, Suzuki Hisanori
Department of Neuroscience and Vision, Section of Biochemistry, University of Verona, Verona, Italy.
Biochem Biophys Res Commun. 2004 Mar 12;315(3):589-94. doi: 10.1016/j.bbrc.2004.01.088.
In this study, alpha-bisabolol, a sesquiterpene alcohol present in natural essential oil, was found to have a strong time- and dose-dependent cytotoxic effect on human and rat glioma cells. After 24 h of treatment with 2.5-3.5 microM alpha-bisabolol, the viability of these cells was reduced by 50% with respect to untreated cells. Furthermore, the viability of normal rat glial cells was not affected by treatment with alpha-bisabolol at the same concentrations as above. Glioma cells treated with high concentration of alpha-bisabolol (10 microM) resulted in a 100% cell death. Judging from hypo-G1 accumulation, poly(ADP-ribose) polymerase cleavage, and DNA ladder formation, the cytotoxicity triggered by alpha-bisabolol resulted from apoptosis induction. Moreover, the dissipation of mitochondrial-inner transmembrane potential and the release of cytochrome c from mitochondria indicated that, in these glioma cells, apoptosis occurred through an intrinsic pathway. As pointed out by the experimental results, alpha-bisabolol may be considered a novel compound able to inhibit glioma cell growth and survival.
在本研究中，发现天然香精油中的倍半萜醇α-红没药醇对人和大鼠胶质瘤细胞具有强烈的时间和剂量依赖性细胞毒性作用。用2.5 - 3.5微摩尔α-红没药醇处理24小时后，这些细胞的活力相对于未处理细胞降低了50%。此外，相同浓度的α-红没药醇处理对正常大鼠神经胶质细胞的活力没有影响。用高浓度α-红没药醇（10微摩尔）处理的胶质瘤细胞导致100%细胞死亡。从G1期前积累、聚（ADP-核糖）聚合酶裂解和DNA梯状条带形成判断，α-红没药醇引发的细胞毒性是由凋亡诱导所致。此外，线粒体内膜跨膜电位的消散和细胞色素c从线粒体的释放表明，在这些胶质瘤细胞中，凋亡是通过内源性途径发生的。实验结果表明，α-红没药醇可被视为一种能够抑制胶质瘤细胞生长和存活的新型化合物。
