Harry I S, Thompson M R, Lu C Y, Thorpe T A
Plant Physiology Research Group, Department of Biology, University of Calgary, Calgary, Alberta T2N 1N4, Canada.
Tree Physiol. 1987 Sep;3(3):273-83. doi: 10.1093/treephys/3.3.273.
A protocol is described for the in vitro production of plantlets from embryonic explants of eastern white cedar (Thuja occidentalis L.). Bud induction was optimal when embryonic explants were cultured for 20-25 days on half-strength Quoirin and Le Poivre mineral salts containing equimolar concentrations (10(-6) M) of N(6)-benzyladenine and 2-isopentyl adenine. Bud development was achieved in phytohormone-free medium in the presence of activated charcoal. Maximal shoot elongation occurred on half-strength Quoirin and Le Poivre salts, whereas shoot multiplication was optimal on half-strength Bornman's MCM salts in the presence of cytokinin. Hardened shoots, dipped in commercial rooting powder containing indole-3-butyric acid, rooted optimally in mist under non-sterile greenhouse conditions. Both rooting and subsequent plantlet growth was best when Redi-Earth((R)) was used as a substrate. Over 250 plantlets per embryo can be produced annually by this technique.
本文描述了一种从东部白松（北美香柏，Thuja occidentalis L.）胚胎外植体体外生产植株的方案。当胚胎外植体在含有等摩尔浓度（10⁻⁶ M）N⁶-苄基腺嘌呤和2-异戊烯腺嘌呤的半强度Quoirin和Le Poivre矿质盐培养基上培养20-25天时，芽诱导效果最佳。在含有活性炭的无植物激素培养基中可实现芽的发育。最大茎伸长发生在半强度Quoirin和Le Poivre盐培养基上，而在细胞分裂素存在下，茎增殖在半强度Bornman's MCM盐培养基上效果最佳。将硬化的茎浸入含有吲哚-3-丁酸的商业生根粉中，在非无菌温室条件下的喷雾环境中生根效果最佳。当使用Redi-Earth®作为基质时，生根和后续植株生长效果最佳。通过该技术，每个胚胎每年可生产超过250株植株。
