Kulchetscki L, Harry I S, Yeung E C, Thorpe T A
Plant Physiology Research Group, Department of Biological Sciences, University of Calgary, Calgary, Alberta T2N 1N4, Canada.
Tree Physiol. 1995 Nov;15(11):727-38. doi: 10.1093/treephys/15.11.727.
A protocol is described for the in vitro propagation of Abies amabilis (Dougl.) Forbes. Over 60% of the cotyledonary explants from 5-day-old cotyledons formed shoots when cultured for 7 days on Schenk and Hildebrandt's (SH) medium containing 10 micro M N(6)-benzyladenine (BA) followed by another 7 days on SH medium containing 10 micro M each of BA and zeatin. Shoot multiplication was unsuccessful. Seventeen percent rooting was obtained after pulsing for 4 h in 1 mM indole-3-butyric acid and planting pulsed shoots in 1/1 (v/v) perlite/vermiculite. Cell clusters (promeristemoids) of five to seven cells were observed on the cotyledonary explants after 7 days of culture in the presence of cytokinin. These cells developed further into meristematic domes and apical meristems. In the absence of cytokinin, stomata and resin canals reached maturity, whereas cells within the cortex became vacuolated and developed into palisade and spongy mesophyll.
本文描述了一种用于美丽冷杉（Abies amabilis (Dougl.) Forbes）离体繁殖的方案。将5日龄子叶的子叶外植体在含有10 μM N(6)-苄基腺嘌呤（BA）的 Schenk和Hildebrandt（SH）培养基上培养7天，然后在含有10 μM BA和玉米素的SH培养基上再培养7天，超过60%的外植体形成了芽。芽的增殖未成功。在1 mM吲哚-3-丁酸中浸泡4小时后，将浸泡过的芽种植在1/1（v/v）珍珠岩/蛭石中，生根率为17%。在细胞分裂素存在的情况下，培养7天后，在子叶外植体上观察到由五到七个细胞组成的细胞团（原分生组织样细胞）。这些细胞进一步发育成分生组织瘤和顶端分生组织。在没有细胞分裂素的情况下，气孔和树脂道成熟，而皮层内的细胞液泡化并发育成栅栏组织和海绵组织。
