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本文引用的文献

1
Organization and expression of eucaryotic split genes coding for proteins.编码蛋白质的真核生物断裂基因的组织与表达。
Annu Rev Biochem. 1981;50:349-83. doi: 10.1146/annurev.bi.50.070181.002025.
2
Selective isolation of cosmid clones by homologous recombination in Escherichia coli.通过大肠杆菌中的同源重组进行黏粒克隆的选择性分离。
Proc Natl Acad Sci U S A. 1984 Jul;81(13):4129-33. doi: 10.1073/pnas.81.13.4129.
3
The TGGCA-binding protein: a eukaryotic nuclear protein recognizing a symmetrical sequence on double-stranded linear DNA.TGGCA结合蛋白：一种识别双链线性DNA上对称序列的真核细胞核蛋白。
Nucleic Acids Res. 1984 May 25;12(10):4295-311. doi: 10.1093/nar/12.10.4295.
4
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.一种将DNA限制性内切酶片段放射性标记至高比活度的技术。
Anal Biochem. 1983 Jul 1;132(1):6-13. doi: 10.1016/0003-2697(83)90418-9.
5
The isoenzymes of glutathione transferase.谷胱甘肽转移酶的同工酶
Adv Enzymol Relat Areas Mol Biol. 1985;57:357-417. doi: 10.1002/9780470123034.ch5.
6
Human liver glutathione S-transferases: complete primary sequence of an Ha subunit cDNA.人肝脏谷胱甘肽S-转移酶：Ha亚基cDNA的完整一级序列
Biochem Biophys Res Commun. 1986 Nov 26;141(1):229-37. doi: 10.1016/s0006-291x(86)80358-8.
7
The separation of glutathione transferase subunits by using reverse-phase high-pressure liquid chromatography.利用反相高压液相色谱法分离谷胱甘肽转移酶亚基。
Biochem J. 1987 Jul 15;245(2):423-8. doi: 10.1042/bj2450423.
8
Mouse glutathione S-transferase Ya subunit: gene structure and sequence.小鼠谷胱甘肽S-转移酶Ya亚基：基因结构与序列
DNA. 1987 Aug;6(4):317-24. doi: 10.1089/dna.1987.6.317.
9
Purification and biochemical characterization of the promoter-specific transcription factor, Sp1.启动子特异性转录因子Sp1的纯化及生化特性分析
Science. 1986 Oct 3;234(4772):47-52. doi: 10.1126/science.3529394.
10
Interaction of a liver-specific nuclear factor with the fibrinogen and alpha 1-antitrypsin promoters.一种肝脏特异性核因子与纤维蛋白原和α1-抗胰蛋白酶启动子的相互作用。
Science. 1987 Oct 30;238(4827):688-92. doi: 10.1126/science.3499668.

与cDNA克隆pGTH2对应的人α谷胱甘肽S-转移酶基因的克隆、测序及特性分析

Cloning, sequencing and characterization of the human alpha glutathione S-transferase gene corresponding to the cDNA clone pGTH2.

作者信息

Klöne A, Hussnätter R, Sies H

机构信息

Institut für Physiologische Chemie I, Heinrich-Heine-Universität Düsseldorf, Germany.

出版信息

Biochem J. 1992 Aug 1;285 ( Pt 3)(Pt 3):925-8. doi: 10.1042/bj2850925.

DOI:10.1042/bj2850925
PMID:1497629
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1132883/
Abstract

The human Alpha glutathione S-transferase gene corresponding to the human liver cDNA clone pGTH2 was isolated from a cosmid genome library. The gene, represented by the clone cosGTH2, spans nearly 12 kb and contains seven exons. The intron/exon borders conform to the standard rules, and an open reading frame is present, starting at position 67 in exon 2, the double-stop codon being at position 733 in exon 7. Exons 1, 2 and 7 differ in length from the known rat gene coding for the Ya enzyme. A 209 bp 5'-upstream region contains TATA and CAT boxes and, in addition, motifs for Sp1-, NF1- and HNFI-binding factors. Clone cosGTH2 represents the less basic subunit, alpha y, of two Alpha glutathione S-transferase subunits (alpha x and alpha y) expressed in liver, which is identical with the kidney subunit alpha 2.

摘要

从黏粒基因组文库中分离出了与人类肝脏cDNA克隆pGTH2对应的人类α谷胱甘肽S-转移酶基因。该基因由克隆cosGTH2代表，跨度近12 kb，包含7个外显子。内含子/外显子边界符合标准规则，存在一个开放阅读框，起始于外显子2的第67位，双终止密码子位于外显子7的第733位。外显子1、2和7的长度与已知的编码Ya酶的大鼠基因不同。一个209 bp的5'-上游区域包含TATA盒和CAT盒，此外还有Sp1、NF1和HNFI结合因子的基序。克隆cosGTH2代表肝脏中表达的两种α谷胱甘肽S-转移酶亚基（αx和αy）中碱性较弱的亚基αy，它与肾脏亚基α2相同。