Maecker Holden T
Immunocytometry Systems, BD Biosciences, San Jose, CA, USA.
Methods Mol Biol. 2004;263:95-108. doi: 10.1385/1-59259-773-4:095.
Cytokine flow cytometry (CFC) is a general term that applies to flow cytometric analysis of cells using anticytokine antibodies as markers of activation. The most common version of this technique is the intracellular staining of cytokines in cells that have been fixed and permeabilized after short-term in vitro activation. When used with specific antigens, this technique allows for the quantitation of rare populations of antigen-specific T cells. In this chapter, specific methodology for such intracellular staining is elaborated, with emphasis on the effects of variables such as sample type, antigens, activation conditions, sample processing, and data acquisition and analysis.
细胞因子流式细胞术（CFC）是一个通用术语，适用于使用抗细胞因子抗体作为激活标志物对细胞进行流式细胞术分析。该技术最常见的形式是对在短期体外激活后进行固定和通透处理的细胞内细胞因子进行染色。当与特定抗原一起使用时，该技术可对罕见的抗原特异性T细胞群体进行定量。在本章中，将详细阐述这种细胞内染色的具体方法，重点是样本类型、抗原、激活条件、样本处理以及数据采集和分析等变量的影响。
