Pahar Bapi, Li Jun, McChesney Michael B
California National Primate Research Center, University of California-Davis, Davis, California 95616, USA.
J Immunol Methods. 2005 Sep;304(1-2):174-83. doi: 10.1016/j.jim.2005.07.018.
A low, average frequency (0.61%) of measles virus (MV)-specific CD4 and CD8+ T cells was detected in rhesus monkeys experimentally infected with or vaccinated against MV. Both IFN-gamma and TNF-alpha positive T cells were visualized by flow cytometry. However, the conditions of short-term culture and stimulation to detect MV-specific T cells required significant modifications from a previously established method that reliably detects T cells in rhesus monkeys persistently infected with SIV. Both whole viral antigen and short synthetic peptide pools were an adequate antigenic stimulus. MV-specific T cells were detectable up to 11 years after exposure to the virus, although we cannot rule out possible subclinical re-exposure of the monkeys to vaccine virus during this time. Thus, flow cytometric methods can permit mechanistic studies of antigen-specific memory T cell dynamics following an acute viral infection in a primate model.
在实验性感染或接种麻疹病毒（MV）的恒河猴中，检测到低频率（0.61%）的MV特异性CD4和CD8+T细胞。通过流式细胞术观察到IFN-γ和TNF-α阳性T细胞。然而，检测MV特异性T细胞所需的短期培养和刺激条件与之前建立的可靠检测持续感染SIV的恒河猴T细胞的方法相比需要显著修改。全病毒抗原和短合成肽库都是足够的抗原刺激。接触病毒后长达11年都可检测到MV特异性T细胞，尽管我们不能排除在此期间猴子可能再次亚临床接触疫苗病毒的情况。因此，流式细胞术方法可用于灵长类动物模型中急性病毒感染后抗原特异性记忆T细胞动力学的机制研究。
