Tesfa L, Volk H D, Kern F
Institut für Medizinische Immunologie, Berliner Hochschulmedizin Charite, Charité Campus Mitte, Berlin, Germany.
J Biol Regul Homeost Agents. 2003 Oct-Dec;17(4):366-70.
Flow-cytometry can be used in different ways in order to analyze or enumerate antigen specific T-cells. The three basic principles are direct staining of the T-cell receptor using so called tetramer reagents, staining intracellular cytokines following antigen-specific ex vivo T-cell activation or staining with dyes that are incorporated (increase in staining) or distributed between daughter cells (decrease in staining) upon proliferation in response to a specific antigen challenge. Each system has its advantages and disadvantages. Here we demonstrate that tetramer staining, cytokine flow cytometry and staining with CFDA-SE can be combined permitting the analysis of proliferation and cytokine production with a subset of T-cells specific for a single peptide antigen.
流式细胞术可通过不同方式用于分析或计数抗原特异性T细胞。三个基本原理是：使用所谓的四聚体试剂直接染色T细胞受体；在抗原特异性体外T细胞激活后染色细胞内细胞因子；或用在响应特定抗原刺激而增殖时被掺入（染色增加）或分布于子代细胞之间（染色减少）的染料进行染色。每个系统都有其优缺点。在此我们证明，四聚体染色、细胞因子流式细胞术和CFDA-SE染色可以结合使用，从而允许对针对单一肽抗原的T细胞亚群的增殖和细胞因子产生进行分析。
