Hirooka Kazuyuki, Tokuda Masaaki, Miyamoto Osamu, Itano Toshifumi, Baba Tetsuya, Shiraga Fumio
Department of Ophthalmology, Kagawa University School of Medicine, Miki, Kagawa, Japan.
Curr Eye Res. 2004 Mar;28(3):153-7. doi: 10.1076/ceyr.28.3.153.26246.
To investigate the effect of Ginkgo biloba extract (EGb 761) against neurotoxicity of retinal ganglion cells of rats with chronic moderately elevated intraocular pressure (IOP).
Unilateral chronic moderately elevated IOP was produced in rats by cautery of three episcleral vessels. Secondary degeneration was measured with and without EGb 761 for 5 months. At 5 months, retinal ganglion cells were labeled with a fast blue tracer applied to both superior colliculi. Densities of surviving retinal ganglion cells were estimated by counting fast blue labeled cells in whole mounted retinas.
When compared with their contralateral control eyes with normal IOP, in the peripheral retina, retinal ganglion cell loss in eyes with chronic, moderately elevated IOP was 29.8 +/- 1.5% (n=5) at 5 months in untreated animals and 4.6 +/- 4.5% (n=5) at 5 months in treated animals with EGb 761.
Pretreatment and early posttreatment with EGb 761 is an effective neuroprotectant in a rat model of chronic glaucoma.
研究银杏叶提取物（EGb 761）对慢性中度眼压升高（IOP）大鼠视网膜神经节细胞神经毒性的影响。
通过烧灼三条巩膜上血管在大鼠中制造单侧慢性中度眼压升高。在使用和不使用EGb 761的情况下测量5个月的继发性变性。在5个月时，将快速蓝色示踪剂应用于双侧上丘以标记视网膜神经节细胞。通过计数全层视网膜中快速蓝色标记的细胞来估计存活的视网膜神经节细胞的密度。
与对侧眼压正常的对照眼相比，在周边视网膜中，未经治疗的动物在5个月时慢性中度眼压升高眼的视网膜神经节细胞损失为29.8±1.5%（n = 5），而用EGb 761治疗的动物在5个月时为4.6±4.5%（n = 5）。
在慢性青光眼大鼠模型中，EGb 761预处理和早期治疗后是一种有效的神经保护剂。
