Hasebe Akira, Yoshimura Atsutoshi, Into Takeshi, Kataoka Hideo, Tanaka Saori, Arakawa Shinichi, Ishikura Hiroaki, Golenbock Douglas T, Sugaya Tsutomu, Tsuchida Nobuo, Kawanami Masamitsu, Hara Yoshitaka, Shibata Ken-Ichiro
Departments of Oral Pathobiological Science. Oral Health Science, Hokkaido University Graduate School of Dental Medicine, Nishi 7, Kita 13, Kita-ku, Sapporo 060-8586, Japan.
Infect Immun. 2004 Mar;72(3):1318-25. doi: 10.1128/IAI.72.3.1318-1325.2004.
Bacteroides forsythus is a gram-negative, anaerobic, fusiform bacterium and is considered to be an etiological agent in periodontal disease. A lipoprotein fraction prepared from B. forsythus cells by Triton X-114 phase separation (BfLP) activated human gingival fibroblasts and a human monocytic cell line, THP-1, to induce interleukin-6 production and tumor necrosis factor alpha production. BfLP was found to be capable of inducing nuclear factor-kappaB translocation in human gingival fibroblasts and THP-1 cells. By using Chinese hamster ovary K1 cells transfected with Toll-like receptor genes together with a nuclear factor-kappaB-dependent CD25 reporter plasmid, it was found that signaling by BfLP was mediated by Toll-like receptor 2 but not by CD14 or Toll-like receptor 4. BfLP induced apoptotic cell death in human gingival fibroblasts, KB cells (an oral epithelial cell line), HL-60 cells (a human myeloid leukemia cell line), and THP-1 cells but not in MOLT4 cells (a T-cell leukemia cell line). Caspase-8, an initiator caspase in apoptosis, was found to be activated in these cells in response to BfLP stimulation. Thus, this study suggested that BfLP plays some etiological roles in oral infections, especially periodontal disease, by induction of cell activation or apoptosis.
福赛斯坦纳菌是一种革兰氏阴性、厌氧、梭形细菌，被认为是牙周病的病原体。通过Triton X-114相分离从福赛斯坦纳菌细胞中制备的脂蛋白组分（BfLP）可激活人牙龈成纤维细胞和人单核细胞系THP-1，以诱导白细胞介素-6和肿瘤坏死因子α的产生。发现BfLP能够诱导人牙龈成纤维细胞和THP-1细胞中的核因子-κB易位。通过使用转染了Toll样受体基因的中国仓鼠卵巢K1细胞以及核因子-κB依赖性CD25报告质粒，发现BfLP的信号传导是由Toll样受体2介导的，而不是由CD14或Toll样受体4介导的。BfLP可诱导人牙龈成纤维细胞、KB细胞（一种口腔上皮细胞系）、HL-60细胞（一种人髓系白血病细胞系）和THP-1细胞发生凋亡性细胞死亡，但对MOLT4细胞（一种T细胞白血病细胞系）无此作用。凋亡起始半胱天冬酶-8在这些细胞中被发现会因BfLP刺激而被激活。因此，本研究表明BfLP通过诱导细胞激活或凋亡在口腔感染尤其是牙周病中发挥一些病因学作用。
