Lee Jung-Hee, Park Hyo-Soon, Jang Won-Jong, Koh Seong-Eun, Park Tae-Kyu, Kang Shin-Seok, Kim Bum-Joon, Kook Yoon-Hoh, Park Kyung-Hee, Lee Seung-Hyun
Department of Microbiology, College of Medicine, Seoul, Korea.
Microbiol Immunol. 2004;48(2):125-30. doi: 10.1111/j.1348-0421.2004.tb03498.x.
Two Haemaphysalis longicornis ticks were found positive in PCR assay of com-1 gene to detect Coxiella burnetii DNA from 100 ticks. The nucleotide sequences of com-1 and 16S rRNA gene were determined from 2 ticks and compared with those of other C. burnetii strains. The results suggest that H. longicornis harbor Coxiella sp. bacteria in Korea. Furthermore, icd, cbhE', and cbbE' genes are C. burnetii specific genes whereas com-1 gene is Coxiella genus specific gene. This study gives the first documentation to prove the existence of Coxiella sp. in tick collected in Korea.
在对100只蜱进行检测伯氏考克斯氏体DNA的com-1基因PCR检测中，发现两只长角血蜱呈阳性。从2只蜱中测定了com-1和16S rRNA基因的核苷酸序列，并与其他伯氏考克斯氏体菌株的序列进行了比较。结果表明，韩国的长角血蜱携带柯克斯体属细菌。此外，icd、cbhE'和cbbE'基因是伯氏考克斯氏体特异性基因，而com-1基因是柯克斯体属特异性基因。本研究首次提供了证据，证明在韩国采集的蜱中存在柯克斯体属细菌。
