Kim Hyun-Yong, Lee Hee Jung, Zuo Guanglei, Hwang Seung Hwan, Park Jeong Seok, Hong Jae Seung, Kim Kang Hyuk, Soto Montero Silvia, Yi Dong-Keun, Lee Jeong Tae, Suh Hong-Won, Lim Soon Sung
Department of Food Science and Nutrition College of Natural Sciences Hallym University Chuncheon Korea.
Department of Pharmacology College of Medicine Hallym University Chuncheon Korea.
Food Sci Nutr. 2022 Apr 7;10(7):2381-2389. doi: 10.1002/fsn3.2846. eCollection 2022 Jul.
Benth. (CE) is native to the Mexico and multiple effects have been observed from several plants belonging to the same family. CE was subjected to extraction with 95% ethanol, and the components were isolated through column chromatography. The structure of the compound was elucidated based on nuclear magnetic resonance (NMR) spectral data, electron ionization-mass (EI-MS) spectroscopy, and liquid chromatography-mass (LC-MS) spectroscopy. In vivo antinociceptive studies were conducted using writhing, 5% formalin, tail-flick, hot-plate, and von Frey filament tests. The ethanolic extract showed a significant effect in the acetic acid-induced pain model and nociceptive behavior in the formalin model (second phase). In hot-plate test and tail-flick test, the results showed no difference compared to the control group. The results suggest that the ethanolic extract may act peripherally to reduce pain. In the streptozotocin (STZ)-induced pain model, the ethanolic extract showed significant effect in the von Frey test model. The -Hex (Hexane) and MC (Methylene chloride) fractions and isolated compounds, ellagic acid and agathisflavone, showed increased effect. Based on these results, we confirmed that the CE ethanolic extract and their compounds, ellagic acid and agathisflavone, have antinociceptive effect on diabetes mellitus-induced pain. Furthermore, the results of this study might be valuable for identifying compounds with antinociceptive activity from natural products.
本特（CE）原产于墨西哥，并且已从同一科的几种植物中观察到多种效应。CE用95%乙醇进行提取，其成分通过柱色谱法分离。该化合物的结构根据核磁共振（NMR）光谱数据、电子电离质谱（EI-MS）光谱和液相色谱-质谱（LC-MS）光谱进行阐明。使用扭体法、5%福尔马林法、甩尾法、热板法和von Frey细丝法进行体内抗伤害感受研究。乙醇提取物在醋酸诱导的疼痛模型和福尔马林模型（第二阶段）的伤害感受行为中显示出显著效果。在热板试验和甩尾试验中，结果与对照组相比无差异。结果表明乙醇提取物可能通过外周作用减轻疼痛。在链脲佐菌素（STZ）诱导的疼痛模型中，乙醇提取物在von Frey试验模型中显示出显著效果。己烷（Hex）和二氯甲烷（MC）馏分以及分离出的化合物鞣花酸和贝壳杉黄酮显示出增强的效果。基于这些结果，我们证实CE乙醇提取物及其化合物鞣花酸和贝壳杉黄酮对糖尿病诱导的疼痛具有抗伤害感受作用。此外，本研究结果对于从天然产物中鉴定具有抗伤害感受活性的化合物可能具有重要价值。
