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Immuno-depletion and purification strategies to study chromatin-remodeling factors in vitro.

作者信息

Chalkley Gillian E, Verrijzer C Peter

机构信息

Gene Regulation Laboratory, Center for Biomedical Genetics, Department of Molecular and Cell Biology, Leiden University Medical Center, 2300 RA Leiden, The Netherlands.

出版信息

Methods Enzymol. 2004;377:421-42. doi: 10.1016/S0076-6879(03)77028-1.

DOI:10.1016/S0076-6879(03)77028-1
PMID:14979043
Abstract
摘要

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Immuno-depletion and purification strategies to study chromatin-remodeling factors in vitro.
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Chromatin remodeling in DNA replication.DNA复制中的染色质重塑
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Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins.当前用于纯化重组蛋白的亲和标签使用及标签去除策略。
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Use of bifunctional cross-linking reagents in mapping genomic distribution of chromatin remodeling complexes.双功能交联试剂在绘制染色质重塑复合物基因组分布中的应用。
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Single step intein-mediated purification of hGMCSF expressed in salt-inducible E. coli.在盐诱导型大肠杆菌中表达的人粒细胞巨噬细胞集落刺激因子(hGMCSF)的单步内含肽介导纯化。
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